Overview
- External ontology
- mediadive.medium:1654 (source link unavailable) — GILLIAMELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1654.pdf
Composition
flowchart LR
CultureMech_001136["gilliamella_medium"]
ing0_Proteose_peptone["Proteose peptone"]
CultureMech_001136 --> ing0_Proteose_peptone
ing1_Beef_extract["Beef extract"]
CultureMech_001136 --> ing1_Beef_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_001136 --> ing2_Yeast_extract
ing3_Malt_extract["Malt extract"]
CultureMech_001136 --> ing3_Malt_extract
ing4_Dextrose["Dextrose"]
CultureMech_001136 --> ing4_Dextrose
CHEBI_17634["CHEBI:17634"]
ing4_Dextrose -.-> CHEBI_17634
ing5_ACES["ACES"]
CultureMech_001136 --> ing5_ACES
CHEBI_39061["CHEBI:39061"]
ing5_ACES -.-> CHEBI_39061
ing6_Agar["Agar"]
CultureMech_001136 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Charcoal["Charcoal"]
CultureMech_001136 --> ing7_Charcoal
CHEBI_91090["CHEBI:91090"]
ing7_Charcoal -.-> CHEBI_91090
ing8_Na_ascorbate["Na-ascorbate"]
CultureMech_001136 --> ing8_Na_ascorbate
CHEBI_113451["CHEBI:113451"]
ing8_Na_ascorbate -.-> CHEBI_113451
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Proteose peptone | 20 G_PER_L | ||
| 2 | Beef extract | 3 G_PER_L | ||
| 3 | Yeast extract | 3 G_PER_L | ||
| 4 | Malt extract | 3 G_PER_L | ||
| 5 |
Dextrose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 6 | ACES | 3.64 G_PER_L |
CHEBI:39061
ACES
|
|
| 7 | Agar | 14 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 8 | Charcoal | 2 G_PER_L |
CHEBI:91090
charcoal
|
|
| 9 |
Na-ascorbate
C6H7O6.Na 198.106 g/mol
|
10 G_PER_L |
CHEBI:113451
sodium ascorbate
|
Preparation
- Add ascorbate solution ( 20 g per litre) after autoclaving and cooling. Final pH of the medium is 7.2. Charcoal should be omitted in liquid medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.137582Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1654 |
| 2026-03-13T06:19:01.527430Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001136 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.256225+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GILLIAMELLA MEDIUM" to "gilliamella_medium" for consistent matching |
| 2026-06-05T07:41:06.361561+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.922354+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:59.104616+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |