Overview
- External ontology
- mediadive.medium:1656 (source link unavailable) — HALANAEROBIUM HYDROGENIFORMANS MEDIUM (SL-HP)
- Applications
-
- Microbial cultivation
- pH
- 11.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1656.pdf
Composition
flowchart LR
CultureMech_001138["halanaerobium_hydrogeniformans_medium_sl_hp"]
ing0_NaCl["NaCl"]
CultureMech_001138 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2CO3["Na2CO3"]
CultureMech_001138 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_K2HPO4["K2HPO4"]
CultureMech_001138 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Yeast_extract["Yeast extract"]
CultureMech_001138 --> ing3_Yeast_extract
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001138 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_L_Cysteine["L-Cysteine"]
CultureMech_001138 --> ing5_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing5_L_Cysteine -.-> CHEBI_17561
ing6_NH4NO3["NH4NO3"]
CultureMech_001138 --> ing6_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing6_NH4NO3 -.-> CHEBI_63038
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001138 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_SiO2["SiO2"]
CultureMech_001138 --> ing8_SiO2
CHEBI_30563["CHEBI:30563"]
ing8_SiO2 -.-> CHEBI_30563
ing9_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001138 --> ing9_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing9_MnSO4_x_H2O -.-> CHEBI_86364
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001138 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001138 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_Resazurin["Resazurin"]
CultureMech_001138 --> ing12_Resazurin
CHEBI_8806["CHEBI:8806"]
ing12_Resazurin -.-> CHEBI_8806
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001138 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_Na3_NTA_x_H2O["Na3-NTA x H2O"]
CultureMech_001138 --> ing14_Na3_NTA_x_H2O
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001138 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_ZnCl2["ZnCl2"]
CultureMech_001138 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_001138 --> ing17_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing17_NiSO4_x_6_H2O -.-> CHEBI_53001
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001138 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001138 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_H3BO3["H3BO3"]
CultureMech_001138 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001138 --> ing21_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
3500.980392 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 3500.0, 0.980392] |
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2000 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
315 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 | Yeast extract | 50 G_PER_L | ||
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
42.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
[Merged 2 duplicates: 37.5, 5.0] |
| 6 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
42.0 G_PER_L |
CHEBI:17561
L-cysteine
|
[Merged 2 duplicates: 30.0, 12.0] |
| 7 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.05 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0085 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
SiO2
O2Si 60.084 g/mol
|
0.0075 G_PER_L |
CHEBI:30563
silicon dioxide
|
|
| 10 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0045 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1022392 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0042, 0.0980392] |
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0998392 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.0018, 0.0980392] |
| 13 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.004 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.94118 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 | Na3-NTA x H2O | 1.59804 G_PER_L | ||
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.627451 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.127451 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.0294118 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0245098 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Collapsed 2 identical duplicates: 0.0245098, 0.0245098] |
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.00980392 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.00980392 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00686275 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Preparation
- Prepare the medium without cysteine hydrochloride, Na2S x 9 H2O and Na2CO3. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the Na2CO3. Autoclave the medium and add from sterile stock solutions cysteine hydrochloride and Na2S x 9 H2O. The final pH should be 11.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.142376Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1656 |
| 2026-01-28T19:48:12.408640Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:48.489441Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:01.543908Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001138 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.274709+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALANAEROBIUM HYDROGENIFORMANS MEDIUM (SL-HP)" to "halanaerobium_hydrogeniformans_medium_sl_hp" for consistent matching |
| 2026-06-05T07:41:07.077402+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.291418+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:09.771539+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |