Overview
- External ontology
- mediadive.medium:1664 (source link unavailable) — Sphaerotilus natans subspec. sulfidivorans medium
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1664.pdf
Composition
flowchart LR
CultureMech_001147["sphaerotilus_natans_subspec_sulfidivorans_medium"]
ing0__NH4_Cl["(NH4)Cl"]
CultureMech_001147 --> ing0__NH4_Cl
CHEBI_31206["CHEBI:31206"]
ing0__NH4_Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_001147 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_001147 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Na2HPO4_x_7_H2O["Na2HPO4 x 7 H2O"]
CultureMech_001147 --> ing3_Na2HPO4_x_7_H2O
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4_x_7_H2O -.-> CHEBI_34683
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001147 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2["CaCl2"]
CultureMech_001147 --> ing5_CaCl2
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2 -.-> CHEBI_3312
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001147 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_Na_lactate["Na-lactate"]
CultureMech_001147 --> ing7_Na_lactate
CHEBI_75228["CHEBI:75228"]
ing7_Na_lactate -.-> CHEBI_75228
ing8_Peptone["Peptone"]
CultureMech_001147 --> ing8_Peptone
ing9_Na2S2SO3["Na2S2SO3"]
CultureMech_001147 --> ing9_Na2S2SO3
ing10_EDTA["EDTA"]
CultureMech_001147 --> ing10_EDTA
CHEBI_4735["CHEBI:4735"]
ing10_EDTA -.-> CHEBI_4735
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001147 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001147 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_001147 --> ing13_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing13_MnSO4_x_4_H2O -.-> CHEBI_86358
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001147 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_H3BO3["H3BO3"]
CultureMech_001147 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Co_NO3_2["Co(NO3)2"]
CultureMech_001147 --> ing16_Co_NO3_2
CHEBI_86209["CHEBI:86209"]
ing16_Co_NO3_2 -.-> CHEBI_86209
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001147 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_Riboflavin["Riboflavin"]
CultureMech_001147 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001147 --> ing19_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing19_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_001147 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001147 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001147 --> ing22_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_Calcium_pantothenate -.-> CHEBI_31345
ing23_Biotin["Biotin"]
CultureMech_001147 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_001147 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_001147 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.009 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.022 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Na2HPO4 x 7 H2O
HO4P.2Na 141.958 g/mol
|
0.035 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.023 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.028 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.00025 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 8 |
Na-lactate
C3H5O3.Na 112.06 g/mol
|
0.2 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 9 | Peptone | 0.5 G_PER_L | ||
| 10 | Na2S2SO3 | 1 G_PER_L | ||
| 11 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.2 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.7 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.002 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.005 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Co(NO3)2
Co.2NO3 182.941 g/mol
|
1 G_PER_L |
CHEBI:86209
cobalt dinitrate
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.1 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.5 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.5 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Dissolve ingredients (except vitamins) in distilled water.
- After sterilization add the 25 µl vitamin solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.168799Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1664 |
| 2026-03-13T06:19:01.636862Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001147 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.379209+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sphaerotilus natans subspec. sulfidivorans medium" to "sphaerotilus_natans_subspec_sulfidivorans_medium" for consistent matching |
| 2026-06-05T07:41:46.179376+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.579446+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |