Overview
- External ontology
- mediadive.medium:1668 (source link unavailable) — MDM (modified dehority medium)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1668.pdf
Composition
flowchart LR
CultureMech_001152["mdm_modified_dehority_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001152 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_001152 --> ing1_Tryptone
ing2_L_Cysteine_HCl["L-Cysteine HCl"]
CultureMech_001152 --> ing2_L_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing2_L_Cysteine_HCl -.-> CHEBI_91247
ing3_Biotin["Biotin"]
CultureMech_001152 --> ing3_Biotin
CHEBI_15956["CHEBI:15956"]
ing3_Biotin -.-> CHEBI_15956
ing4_Folic_acid["Folic acid"]
CultureMech_001152 --> ing4_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing4_Folic_acid -.-> CHEBI_27470
ing5_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001152 --> ing5_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing5_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing6_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001152 --> ing6_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing6_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing7_Riboflavin["Riboflavin"]
CultureMech_001152 --> ing7_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing7_Riboflavin -.-> CHEBI_17015
ing8_Nicotinic_acid["Nicotinic acid"]
CultureMech_001152 --> ing8_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing8_Nicotinic_acid -.-> CHEBI_15940
ing9_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_001152 --> ing9_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing9_D_Calcium_pantothenate -.-> CHEBI_31345
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_001152 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001152 --> ing11_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
ing12_Lipoic_acid["Lipoic acid"]
CultureMech_001152 --> ing12_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing12_Lipoic_acid -.-> CHEBI_16494
ing13_Cellobiose["Cellobiose"]
CultureMech_001152 --> ing13_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing13_Cellobiose -.-> CHEBI_17057
ing14_D_Glucose["D-Glucose"]
CultureMech_001152 --> ing14_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing14_D_Glucose -.-> CHEBI_17634
ing15_Maltose["Maltose"]
CultureMech_001152 --> ing15_Maltose
CHEBI_17306["CHEBI:17306"]
ing15_Maltose -.-> CHEBI_17306
ing16_Starch["Starch"]
CultureMech_001152 --> ing16_Starch
CHEBI_28017["CHEBI:28017"]
ing16_Starch -.-> CHEBI_28017
ing17_Ethanol["Ethanol"]
CultureMech_001152 --> ing17_Ethanol
CHEBI_16236["CHEBI:16236"]
ing17_Ethanol -.-> CHEBI_16236
ing18_Vitamin_K1["Vitamin K1"]
CultureMech_001152 --> ing18_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing18_Vitamin_K1 -.-> CHEBI_18067
ing19_K2HPO4["K2HPO4"]
CultureMech_001152 --> ing19_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing19_K2HPO4 -.-> CHEBI_131527
ing20_NaHCO3["NaHCO3"]
CultureMech_001152 --> ing20_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing20_NaHCO3 -.-> CHEBI_32139
ing21_NaCl["NaCl"]
CultureMech_001152 --> ing21_NaCl
CHEBI_26710["CHEBI:26710"]
ing21_NaCl -.-> CHEBI_26710
ing22_NH4Cl["NH4Cl"]
CultureMech_001152 --> ing22_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing22_NH4Cl -.-> CHEBI_31206
ing23_CaCl2["CaCl2"]
CultureMech_001152 --> ing23_CaCl2
CHEBI_3312["CHEBI:3312"]
ing23_CaCl2 -.-> CHEBI_3312
ing24_MgCl2["MgCl2"]
CultureMech_001152 --> ing24_MgCl2
CHEBI_6636["CHEBI:6636"]
ing24_MgCl2 -.-> CHEBI_6636
ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001152 --> ing25_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
ing26_MgCl2_x_2_H2O["MgCl2 x 2 H2O"]
CultureMech_001152 --> ing26_MgCl2_x_2_H2O
CHEBI_131394["CHEBI:131394"]
ing26_MgCl2_x_2_H2O -.-> CHEBI_131394
ing27_ZnCl2["ZnCl2"]
CultureMech_001152 --> ing27_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing27_ZnCl2 -.-> CHEBI_49976
ing28_CoCl2["CoCl2"]
CultureMech_001152 --> ing28_CoCl2
CHEBI_35696["CHEBI:35696"]
ing28_CoCl2 -.-> CHEBI_35696
ing29_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
CultureMech_001152 --> ing29_Na2_MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing29_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
CultureMech_001152 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | Tryptone | 1 G_PER_L | ||
| 3 |
L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
|
1 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 4 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 5 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 6 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 7 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 8 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 9 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 10 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 11 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 13 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 14 |
Cellobiose
C12H22O11 342.297 g/mol
|
101.0 G_PER_L |
CHEBI:17057
cellobiose
|
[Merged 2 duplicates: 100.0, 1.0] |
| 15 |
D-Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 16 |
Maltose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17306
maltose
|
|
| 17 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 18 |
Ethanol
C2H6O 46.069 g/mol
|
19 G_PER_L |
CHEBI:16236
ethanol
|
|
| 19 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 20 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6.804 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 21 |
NaHCO3
CHO3.Na 84.006 g/mol
|
6.721 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 22 |
NaCl
Cl.Na 58.443 g/mol
|
4.675 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 23 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.605 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 24 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.089 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 25 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.095 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 26 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.028 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 27 |
MgCl2 x 2 H2O
2Cl.2H2O.Mg 131.241 g/mol
|
0.971 G_PER_L |
CHEBI:131394
magnesium dichloride dihydrate
|
|
| 28 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.273 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 29 |
CoCl2
2Cl.Co 129.839 g/mol
|
0.104 G_PER_L |
CHEBI:35696
cobalt dichloride
|
|
| 30 |
Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.048 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 31 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.035 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 32 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.237 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 33 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.066 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- To make medium anoxic boil it, cool under N2CO2 gas atmosphere, add 1 g/l L-cysteine hydrochloride and adjust pH to 6.8. Finally gas over with N2 . Dispense under N2CO2 into suitable vessels for anaerobic growth (serum bottles or Hungate tubes sealed with rubber stoppers) and sterilize by autoclaving.
- Filter sterilize and make anoxic with N2.
- Mix 10 g cellobiose with 100 ml distilled water and filter sterilize, make anoxic with N2.
- Make anoxic with N2.
- Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Make anoxic with N2. Store refrigerated in a brown bottle.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.188338Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1668 |
| 2026-01-28T19:48:34.186346Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:28.301851Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:01.697884Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001152 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.448541+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MDM (modified dehority medium)" to "mdm_modified_dehority_medium" for consistent matching |
| 2026-06-05T07:41:13.502069+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.075286+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |