Overview
- External ontology
- mediadive.medium:1669 (source link unavailable) — SCHAEDLER BROTH (Roth; 5772)
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1669.pdf
Composition
flowchart LR
CultureMech_001153["schaedler_broth_roth_5772"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_001153 --> ing0_Casein_peptone
ing1_Soy_peptone["Soy peptone"]
CultureMech_001153 --> ing1_Soy_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001153 --> ing2_Yeast_extract
ing3_Peptone_mixture["Peptone mixture"]
CultureMech_001153 --> ing3_Peptone_mixture
ing4_Glucose["Glucose"]
CultureMech_001153 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_K2HPO4["K2HPO4"]
CultureMech_001153 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_NaCl["NaCl"]
CultureMech_001153 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Tris["Tris"]
CultureMech_001153 --> ing7_Tris
CHEBI_9754["CHEBI:9754"]
ing7_Tris -.-> CHEBI_9754
ing8_Resazurin["Resazurin"]
CultureMech_001153 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Hemin["Hemin"]
CultureMech_001153 --> ing9_Hemin
CHEBI_50385["CHEBI:50385"]
ing9_Hemin -.-> CHEBI_50385
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001153 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 5.66 G_PER_L | ||
| 2 | Soy peptone | 1 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 | Peptone mixture | 5 G_PER_L | ||
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
5.83 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.83 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
1.66 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
Tris
C4H11NO3 121.136 g/mol
|
3 G_PER_L |
CHEBI:9754
tris
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
0.01 G_PER_L |
CHEBI:50385
hemin
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.4 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Boil medium under CO2 until the resazurin indicator changes from pink to colourless. Cool down under CO2 and adjust pH to 7.6. Switch to N2 to prevent further lowering of the pH. Fill up into Hungate tubes under N2 and autoclave.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.193168Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1669 |
| 2026-03-13T06:19:01.706638Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001153 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.458678+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SCHAEDLER BROTH (Roth; 5772)" to "schaedler_broth_roth_5772" for consistent matching |
| 2026-06-05T07:41:45.360774+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.333992+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |