bme_ctvm2_cell_line_medium_occidentia_massiliensis

Overview

External ontology

mediadive.medium:1670 (source link unavailable) — BME/CTVM2 cell line medium (Occidentia massiliensis)

Applications

• Microbial cultivation

Notes

DSMZ Medium 1670 prepares a 101 ml BME/CTVM2 tick-cell medium for Occidentia massiliensis cultivation from 70 ml L-15 (Leibovitz) medium, 10 ml tryptose phosphate broth, 20 ml foetal calf serum, and 1 ml 200 mM L-glutamine stock.

Composition

flowchart LR
    CultureMech_001155["bme_ctvm2_cell_line_medium_occidentia_massiliensis"]
    ing0_L_15__Leibovitz__medium["L-15 (Leibovitz) medium"]
    CultureMech_001155 --> ing0_L_15__Leibovitz__medium
    ing1_Tryptose_phosphate_broth["Tryptose phosphate broth"]
    CultureMech_001155 --> ing1_Tryptose_phosphate_broth
    ing2_Foetal_calf_serum["Foetal calf serum"]
    CultureMech_001155 --> ing2_Foetal_calf_serum

Ingredients (3)

Preparation

1. Prepare the BME/CTVM2 cell-culture medium from 70 ml L-15 (Leibovitz) medium, 10 ml tryptose phosphate broth, 20 ml foetal calf serum, and 1 ml 200 mM L-glutamine.
2. Use the medium for BME/CTVM2 cells incubated in ordinary air in sealed flasks or flat-sided tubes in dry incubators.
3. For Occidentia massiliensis infection, add 3 ml fresh medium and 1 ml thawed Occidentia strain to a 25 cm2 flask with 5 ml residual medium, then incubate at 28 C with the lid closed and without CO2.

Solutions

Target organisms

No target organisms are recorded.

Provenance