Overview
- External ontology
- mediadive.medium:1673 (source link unavailable) — Cyanobacteria Medium ASN 3+
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1673.pdf
Composition
flowchart LR
CultureMech_001157["cyanobacteria_medium_asn_3"]
ing0_NaCl["NaCl"]
CultureMech_001157 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001157 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_001157 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Mg_SO4__x_7_H2O["Mg(SO4) x 7 H2O"]
CultureMech_001157 --> ing3_Mg_SO4__x_7_H2O
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001157 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na3_citrate_x_2_H2O["Na3-citrate x 2 H2O"]
CultureMech_001157 --> ing5_Na3_citrate_x_2_H2O
CHEBI_32142["CHEBI:32142"]
ing5_Na3_citrate_x_2_H2O -.-> CHEBI_32142
ing6_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_001157 --> ing6_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing6_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing7_NaNO3["NaNO3"]
CultureMech_001157 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001157 --> ing8_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing9_Na2CO3["Na2CO3"]
CultureMech_001157 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Fe_NH4_citrate["Fe(NH4)citrate"]
CultureMech_001157 --> ing10_Fe_NH4_citrate
CHEBI_31604["CHEBI:31604"]
ing10_Fe_NH4_citrate -.-> CHEBI_31604
ing11_Vitamin_B12["Vitamin B12"]
CultureMech_001157 --> ing11_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing11_Vitamin_B12 -.-> CHEBI_176843
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001157 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001157 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_001157 --> ing14_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing14_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 | Mg(SO4) x 7 H2O | 3.5 G_PER_L | ||
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Na3-citrate x 2 H2O
C6H5O7.2H2O.3Na 294.099 g/mol
|
0.003 G_PER_L |
CHEBI:32142
sodium citrate dihydrate
|
|
| 7 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.0005 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
0.75 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 9 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.02 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.02 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 | Fe(NH4)citrate | 0.003 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 12 | Vitamin B12 | 2e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
10 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Preparation
- Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
- Autoclave at 121 °C for 20 min.
- After cooling, add the following filter-sterilized components:
- Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
Solutions
Trace metal mix
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| H3BO3 | 2.9 G_PER_L | |
| MnCl2 x 4 H2O | 1.81 G_PER_L | |
| CuSO4 x 5 H2O | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.200888Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1673 |
| 2026-01-28T19:48:20.404673Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:01.735699Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001157 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.490421+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Cyanobacteria Medium ASN 3+" to "cyanobacteria_medium_asn_3" for consistent matching |
| 2026-06-05T07:40:55.069260+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.056314+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:21.799568+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace metal mix' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |