Overview
- External ontology
- mediadive.medium:167 (source link unavailable) — SPOROCYTOPHAGA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium167.pdf
Composition
flowchart LR
CultureMech_001161["sporocytophaga_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_001161 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001161 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KCl["KCl"]
CultureMech_001161 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001161 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_KH2PO4["KH2PO4"]
CultureMech_001161 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_001161 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Yeast_extract["Yeast extract"]
CultureMech_001161 --> ing6_Yeast_extract
ing7_Agar["Agar"]
CultureMech_001161 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
2 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.006 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 | Yeast extract | 0.02 G_PER_L | ||
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient |
Preparation
- Adjust pH to 7.2.
- Place a strip of sterile cellulose filter paper onto an agar plate. Alternatively, place a strip of sterile cellulose filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. Inoculate the strain onto the cellulose filter paper. The strain will grow on the cellulose paper only.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.388722Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 167 |
| 2026-03-13T06:19:01.773902Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001161 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.533687+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPOROCYTOPHAGA MEDIUM" to "sporocytophaga_medium" for consistent matching |
| 2026-06-05T07:41:46.618912+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.689448+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |