pes_provasoli_s_enriched_seawater_medium_7_psu_at_25_c

CultureMech:001163 bacterial COMPLEX LIQUID
Original name: PES - Provasoli´s Enriched Seawater-Medium (7 PSU at 25 °C)

Overview

External ontology
mediadive.medium:1680a (source link unavailable) — PES - Provasoli´s Enriched Seawater-Medium (7 PSU at 25 °C)
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001163["pes_provasoli_s_enriched_seawater_medium_7_psu_at_25_c"]
    ing0_Artificial_Sea_Salt["Artificial Sea Salt"]
    CultureMech_001163 --> ing0_Artificial_Sea_Salt
    ing1_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001163 --> ing1_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing1_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing2_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001163 --> ing2_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing2_MnSO4_x_H2O -.-> CHEBI_86364
    ing3_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001163 --> ing3_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing3_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing4_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_001163 --> ing4_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing4_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing5_FeCl3["FeCl3"]
    CultureMech_001163 --> ing5_FeCl3
    CHEBI_30808["CHEBI:30808"]
    ing5_FeCl3 -.-> CHEBI_30808
    ing6_H3BO3["H3BO3"]
    CultureMech_001163 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_NaNO3["NaNO3"]
    CultureMech_001163 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001163 --> ing8_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing9_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
    CultureMech_001163 --> ing9_Sodium_beta_glycerophosphate
    CHEBI_132089["CHEBI:132089"]
    ing9_Sodium_beta_glycerophosphate -.-> CHEBI_132089
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_001163 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_Biotin["Biotin"]
    CultureMech_001163 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Thiamine["Thiamine"]
    CultureMech_001163 --> ing12_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing12_Thiamine -.-> CHEBI_18385

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Artificial Sea Salt 8.2 G_PER_L
2 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
5 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
3 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
100 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
4 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
100 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
5 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
100 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
6 FeCl3
Cl3Fe 162.204 g/mol
100 G_PER_L CHEBI:30808
iron trichloride
7 H3BO3
H3BO3 61.833 g/mol
100 G_PER_L CHEBI:33118
boric acid
8 NaNO3
NO3.Na 84.994 g/mol
100 G_PER_L CHEBI:63005
sodium nitrate
9 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
100 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
10 Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
100 G_PER_L CHEBI:132089
sodium glycerol 2-phosphate
11 Vitamin B12 160 G_PER_L CHEBI:176843
vitamin B12
12 Biotin
C10H16N2O3S 244.316 g/mol
160 G_PER_L CHEBI:15956
biotin
13 Thiamine
C12H17N4OS 265.362 g/mol
800 G_PER_L CHEBI:18385
thiamine(1+)

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling add the following filter sterilized component under sterile conditions:
  4. Make up to <span class='amount' data-volume='500'>500</span> ml with MilliQ water.
  5. Make up to <span class='amount' data-volume='500'>500</span> ml with MilliQ water.
  6. Make up to <span class='amount' data-volume='100'>100</span> ml with MilliQ water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.217113Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1680a
2026-01-28T19:48:37.152087Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:01.788645Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001163
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.549730+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PES - Provasoli´s Enriched Seawater-Medium (7 PSU at 25 °C)" to "pes_provasoli_s_enriched_seawater_medium_7_psu_at_25_c" for consistent matching
2026-06-05T07:41:41.378470+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.045682+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.