mineral_salt_medium_with_colloidal_chitin

CultureMech:001164 bacterial COMPLEX LIQUID
Original name: Mineral salt medium with colloidal chitin

Overview

External ontology
mediadive.medium:1681 (source link unavailable) — Mineral salt medium with colloidal chitin
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1681.pdf

Composition

flowchart LR
    CultureMech_001164["mineral_salt_medium_with_colloidal_chitin"]
    ing0_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001164 --> ing0_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing0_Sodium_resazurin -.-> CHEBI_8806
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001164 --> ing1_Yeast_extract
    ing2_Polypeptone["Polypeptone"]
    CultureMech_001164 --> ing2_Polypeptone
    ing3_NaHCO3["NaHCO3"]
    CultureMech_001164 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Colloidal_chitin["Colloidal chitin"]
    CultureMech_001164 --> ing4_Colloidal_chitin
    ing5_KH2PO4["KH2PO4"]
    CultureMech_001164 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_001164 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001164 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001164 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_K2HPO4["K2HPO4"]
    CultureMech_001164 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_Biotin["Biotin"]
    CultureMech_001164 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_001164 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001164 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001164 --> ing13_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing13_Thiamine_HCl -.-> CHEBI_49105
    ing14_Riboflavin["Riboflavin"]
    CultureMech_001164 --> ing14_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing14_Riboflavin -.-> CHEBI_17015
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001164 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001164 --> ing16_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing17_Vitamin_B12["Vitamin B12"]
    CultureMech_001164 --> ing17_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing17_Vitamin_B12 -.-> CHEBI_176843
    ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001164 --> ing18_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing19__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_001164 --> ing19__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing19__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001164 --> ing20_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing21_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001164 --> ing21_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing21_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing22_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001164 --> ing22_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing22_MnSO4_x_H2O -.-> CHEBI_86364
    ing23_NaCl["NaCl"]
    CultureMech_001164 --> ing23_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing23_NaCl -.-> CHEBI_26710
    ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001164 --> ing24_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001164 --> ing25_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001164 --> ing26_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001164 --> ing27_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing28_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001164 --> ing28_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing28_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing29_H3BO3["H3BO3"]
    CultureMech_001164 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_001164 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 Sodium resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
2 Yeast extract 1 G_PER_L
3 Polypeptone 10 G_PER_L
4 NaHCO3
CHO3.Na 84.006 g/mol
3.52 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 Colloidal chitin 5 G_PER_L
6 KH2PO4
H2O4P.K 136.084 g/mol
8 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 NH4Cl
Cl.H4N 53.492 g/mol
5 G_PER_L CHEBI:31206
ammonium chloride
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 1.0, 0.1]
10 K2HPO4
HO4P.2K 174.174 g/mol
8 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
11 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
12 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
13 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
14 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
15 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
16 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
17 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
18 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
19 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
20 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
21 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
22 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
23 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
24 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
25 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
26 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
27 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
28 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
29 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
30 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
31 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
32 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
33 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
34 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. <b>Reducing agents</b>: Flush each solution under N2 for 30 minutes, stopper and autoclave. L-Cysteine-HCL x H2O solution (10%): add 12.5 µl per 10 ml medium Na2S x 9 H2O solution (3%): add 40 µl per 10 ml medium.
  2. <b>Colloidal chitin preparation</b>: Cut 5 gram commercial crab (shrimp) shell flakes into small pieces, and dissolve in 100 ml of 12M HCL while stirring in a fume hood at room temperature. Add chitin-HCL mixture slowly into pre-cooled water to obtain the colloidal chitin, centrifuge and wash in pre-cooled water till the colloidal chitin reaches a pH of 6 to 6.5. Mix 0.5% with MS medium as the sole carbon source. As an alternative commercial available chitin powder can be used.
  3. <b>Prepare MS medium with chitin</b>: Dissolve the ingredients of MS medium (except NaHCO3 and vitamins), boil medium under CO2 until the resazurin indicator changes from pink to colourless. Cool down under CO2, add NaHCO3 and adjust pH to 7.0. Switch to N2 to prevent further lowering of the pH. Fill up into Hungate tubes under N2 and autoclave. Before use add vitamins (sterilized by filtration) and reducing agents.
  4. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.220187Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1681
2026-02-06T05:10:52.758494Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:40.351173Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:01.801802Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001164
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.563704+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Mineral salt medium with colloidal chitin" to "mineral_salt_medium_with_colloidal_chitin" for consistent matching
2026-06-05T07:41:34.530303+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.027455+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.