cm1a_halorubrum_medium

CultureMech:001165 archaea COMPLEX LIQUID
Original name: CM1A Halorubrum Medium

Overview

External ontology
mediadive.medium:1683 (source link unavailable) — CM1A Halorubrum Medium
Applications
  • Microbial cultivation
pH
7.5
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1683.pdf

Composition

flowchart LR
    CultureMech_001165["cm1a_halorubrum_medium"]
    ing0_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_001165 --> ing0_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing0_Sodium_pyruvate -.-> CHEBI_50144
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001165 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001165 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaCl["NaCl"]
    CultureMech_001165 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001165 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001165 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_H2O["CaCl2 x H2O"]
    CultureMech_001165 --> ing6_CaCl2_x_H2O
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2_x_H2O -.-> CHEBI_3312
    ing7_Salt_water["Salt water"]
    CultureMech_001165 --> ing7_Salt_water
    ing8_Peptone["Peptone"]
    CultureMech_001165 --> ing8_Peptone
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_001165 --> ing9_Yeast_extract
    ing10_HCl["HCl"]
    CultureMech_001165 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001165 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_001165 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001165 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_001165 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001165 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001165 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001165 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001165 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001165 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Biotin["Biotin"]
    CultureMech_001165 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001165 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001165 --> ing22_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing23_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_001165 --> ing23_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing23_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing24_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_001165 --> ing24_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_hydrochloride -.-> CHEBI_49105
    ing25_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_001165 --> ing25_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing25_Cyanocobalamin -.-> CHEBI_17439
    ing26_DL_6_8_thioctic_acid["DL-6,8-thioctic acid"]
    CultureMech_001165 --> ing26_DL_6_8_thioctic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26_DL_6_8_thioctic_acid -.-> CHEBI_16494
    ing27_Riboflavin["Riboflavin"]
    CultureMech_001165 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Folic_acid["Folic acid"]
    CultureMech_001165 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
5.5 G_PER_L CHEBI:50144
sodium pyruvate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.26745 G_PER_L CHEBI:31206
ammonium chloride
3 K2HPO4
HO4P.2K 174.174 g/mol
0.174176 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
30 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
35 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 CaCl2 x H2O
Ca.2Cl 110.984 g/mol
0.74 G_PER_L CHEBI:3312
calcium dichloride
8 Salt water 76.6 G_PER_L
9 Peptone 5 G_PER_L
10 Yeast extract 1 G_PER_L
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.04 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 Biotin
C10H16N2O3S 244.316 g/mol
0.01 G_PER_L CHEBI:15956
biotin
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.1 G_PER_L CHEBI:15940
nicotinic acid
23 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
24 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.15 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
25 Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
0.05 G_PER_L CHEBI:17439
cyanocob(III)alamin
27 DL-6,8-thioctic acid
C8H14O2S2 206.332 g/mol
0.01 G_PER_L CHEBI:16494
lipoic acid
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid

Preparation

  1. Adjust pH to 7.5 with 1 M HCl or 1 M NaOH
  2. Autoclave at 121 °C for 20 min.
  3. Before inoculation add from sterile stock solutions:
  4. Dissolve salts completely then add CaCl2 x H2O.
  5. Adjust pH to 7.5 with Tris-HCl at pH=7.5
  6. Don't use Difco Bacto-peptone, it was reported in 1988 to contain bile salts that lyse halobacteria, this was still the case in 2001
  7. Adjust pH to 7.5
  8. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  9. filter sterilized
  10. filter sterilized
  11. mix 0.25 ml Vit 1 and 0.75 ml Vit2

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.226907Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1683
2026-02-06T05:10:17.982471Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:01.816956Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001165
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.579939+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CM1A Halorubrum Medium" to "cm1a_halorubrum_medium" for consistent matching
2026-06-05T07:40:54.500193+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.912659+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.