Overview
- External ontology
- mediadive.medium:1684 (source link unavailable) — Alicyclobacillus cellulosilyticus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1684.pdf
Composition
flowchart LR
CultureMech_001166["alicyclobacillus_cellulosilyticus_medium"]
ing0_Carboxymethylcellulose["Carboxymethylcellulose"]
CultureMech_001166 --> ing0_Carboxymethylcellulose
CHEBI_85146["CHEBI:85146"]
ing0_Carboxymethylcellulose -.-> CHEBI_85146
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_001166 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_KH2PO4["KH2PO4"]
CultureMech_001166 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Glucose["Glucose"]
CultureMech_001166 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Yeast_extract["Yeast extract"]
CultureMech_001166 --> ing4_Yeast_extract
ing5_Tryptone["Tryptone"]
CultureMech_001166 --> ing5_Tryptone
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001166 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001166 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001166 --> ing8_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing8_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Carboxymethylcellulose | 2.5 G_PER_L |
CHEBI:85146
carboxymethylcellulose
|
(sodium salt) |
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.37 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Yeast extract | 0.25 G_PER_L | ||
| 6 | Tryptone | 0.9 G_PER_L | ||
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Adjust to pH 4.8-5.0 (liquid medium) and pH 5.5 - 6.0 agar medium. Autoclave MgSO4 x 7 H2O solution, CaCl2 x 2 H2O solution and FeCl3 x 6 H2O solution separately.
- Aseptically add all solutions to the basal medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.231404Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1684 |
| 2026-03-13T06:19:01.825167Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001166 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.589080+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alicyclobacillus cellulosilyticus Medium" to "alicyclobacillus_cellulosilyticus_medium" for consistent matching |
| 2026-06-05T07:40:47.757892+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.751951+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |