Overview
- External ontology
- mediadive.medium:1685 (source link unavailable) — HALORUSSUS VARIUS MEDIUM (MSC 15.2)
- Applications
-
- Microbial cultivation
- pH
- 6.9
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1685.pdf
Composition
flowchart LR
CultureMech_001167["halorussus_varius_medium_msc_15_2"]
ing0_NaCl["NaCl"]
CultureMech_001167 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001167 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_H2O["CaCl2 x H2O"]
CultureMech_001167 --> ing2_CaCl2_x_H2O
CHEBI_3312["CHEBI:3312"]
ing2_CaCl2_x_H2O -.-> CHEBI_3312
ing3_KNO3["KNO3"]
CultureMech_001167 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
CultureMech_001167 --> ing4_MgSO4_x_6_H2O
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4_x_6_H2O -.-> CHEBI_32599
ing5_CaCO3["CaCO3"]
CultureMech_001167 --> ing5_CaCO3
CHEBI_3311["CHEBI:3311"]
ing5_CaCO3 -.-> CHEBI_3311
ing6_KBr["KBr"]
CultureMech_001167 --> ing6_KBr
CHEBI_32030["CHEBI:32030"]
ing6_KBr -.-> CHEBI_32030
ing7_NaNO3["NaNO3"]
CultureMech_001167 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001167 --> ing8_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing8_SrCl2_x_6_H2O -.-> CHEBI_36385
ing9_NH4Cl["NH4Cl"]
CultureMech_001167 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_KH2PO4["KH2PO4"]
CultureMech_001167 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_Glucose["Glucose"]
CultureMech_001167 --> ing11_Glucose
CHEBI_17234["CHEBI:17234"]
ing11_Glucose -.-> CHEBI_17234
ing12_Yeast_extract["Yeast extract"]
CultureMech_001167 --> ing12_Yeast_extract
ing13_Agar["Agar"]
CultureMech_001167 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001167 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_Na_EDTA["Na-EDTA"]
CultureMech_001167 --> ing15_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing15_Na_EDTA -.-> CHEBI_64734
ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001167 --> ing16_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
ing17_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001167 --> ing17_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing17_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing18_Na2SeO4_x_10_H2O["Na2SeO4 x 10 H2O"]
CultureMech_001167 --> ing18_Na2SeO4_x_10_H2O
CHEBI_77775["CHEBI:77775"]
ing18_Na2SeO4_x_10_H2O -.-> CHEBI_77775
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001167 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001167 --> ing20_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing20_MnSO4_x_H2O -.-> CHEBI_86364
ing21_Na2MoO4["Na2MoO4"]
CultureMech_001167 --> ing21_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing21_Na2MoO4 -.-> CHEBI_75215
ing22_Na2WO4["Na2WO4"]
CultureMech_001167 --> ing22_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing22_Na2WO4 -.-> CHEBI_63940
ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001167 --> ing23_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing24_AlCl3["AlCl3"]
CultureMech_001167 --> ing24_AlCl3
CHEBI_30114["CHEBI:30114"]
ing24_AlCl3 -.-> CHEBI_30114
ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001167 --> ing25_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
ing26_H3BO3["H3BO3"]
CultureMech_001167 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_CuSO4["CuSO4"]
CultureMech_001167 --> ing27_CuSO4
CHEBI_23414["CHEBI:23414"]
ing27_CuSO4 -.-> CHEBI_23414
ing28_MnCl2["MnCl2"]
CultureMech_001167 --> ing28_MnCl2
CHEBI_63041["CHEBI:63041"]
ing28_MnCl2 -.-> CHEBI_63041
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
210.21 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
23.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x H2O
Ca.2Cl 110.984 g/mol
|
21.7 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 4 |
KNO3
K.NO3 101.102 g/mol
|
18.7 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 5 |
MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
|
4.49 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.015 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 7 |
KBr
Br.K 119.002 g/mol
|
2.6 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
9.44 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 9 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.63 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.05 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.01 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 13 | Yeast extract | 0.1 G_PER_L | ||
| 14 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.54 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
2.06 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 17 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 18 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.2 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 19 |
Na2SeO4 x 10 H2O
2Na.O4Se 188.936 g/mol
|
0.44 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.18 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.12 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 22 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.16 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 23 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.1 G_PER_L |
CHEBI:63940
sodium tungstate
|
|
| 24 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.54 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 25 |
AlCl3
AlCl3 133.341 g/mol
|
0.04 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 26 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.11 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
CuSO4
Cu.O4S 159.609 g/mol
|
0.21 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 29 |
MnCl2
Cl2Mn 125.844 g/mol
|
0.19 G_PER_L |
CHEBI:63041
manganese(II) chloride
|
Preparation
- Adjust the pH 6.9
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
- Final pH 7.0 (with KOH).
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- HALORUSSUS VARIUS
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.233430Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1685 |
| 2026-01-28T19:48:06.690919Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:11.843839Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-02-20T07:49:55.000000Z | organism-data-backfill | Added curated organism data | Added organism: HALORUSSUS VARIUS, culture_type: isolate |
| 2026-03-13T06:19:01.836135Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001167 |
| 2026-03-17T06:29:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.600499+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALORUSSUS VARIUS MEDIUM (MSC 15.2)" to "halorussus_varius_medium_msc_15_2" for consistent matching |
| 2026-06-05T07:41:08.187549+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.969983+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:59.655311+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 10 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |