Overview
- External ontology
- mediadive.medium:1688 (source link unavailable) — ACTINOBACTERIA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1688.pdf
Composition
flowchart LR
CultureMech_001170["actinobacteria_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001170 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2SO4["Na2SO4"]
CultureMech_001170 --> ing1_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing1_Na2SO4 -.-> CHEBI_32149
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_001170 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001170 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_001170 --> ing4_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing5_KCl["KCl"]
CultureMech_001170 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Yeast_extract["Yeast extract"]
CultureMech_001170 --> ing6_Yeast_extract
ing7_Glucose["Glucose"]
CultureMech_001170 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.15 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.45 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.015 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.05 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 | Yeast extract | 0.1 G_PER_L | ||
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
0.9 G_PER_L |
CHEBI:17234
glucose
|
Preparation
- Adjust the pH to 3.0 with H2SO4 or HCl.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.243358Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1688 |
| 2026-03-13T06:19:01.861762Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001170 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.629171+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACTINOBACTERIA MEDIUM" to "actinobacteria_medium" for consistent matching |
| 2026-06-05T07:40:47.476319+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.663411+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:57:59.694168+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |