artificial_sea_water_tryptone_soy_broth_medium_asw_tsb_medium

CultureMech:001173 bacterial COMPLEX SOLID_AGAR
Original name: Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium)

Overview

External ontology
mediadive.medium:1691 (source link unavailable) — Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium)
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1691.pdf

Composition

flowchart LR
    CultureMech_001173["artificial_sea_water_tryptone_soy_broth_medium_asw_tsb_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001173 --> ing0_Trypticase_peptone
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_001173 --> ing1_Soy_peptone
    ing2_D_Glucose["D-Glucose"]
    CultureMech_001173 --> ing2_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D_Glucose -.-> CHEBI_17634
    ing3_Agar["Agar"]
    CultureMech_001173 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_NaCl["NaCl"]
    CultureMech_001173 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001173 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001173 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_KCl["KCl"]
    CultureMech_001173 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001173 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001173 --> ing9_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing9_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing10_NH4NO3["NH4NO3"]
    CultureMech_001173 --> ing10_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing10_NH4NO3 -.-> CHEBI_63038
    ing11_Double_distilled_water["Double distilled water"]
    CultureMech_001173 --> ing11_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Double_distilled_water -.-> CHEBI_15377
    ing12_HCl["HCl"]
    CultureMech_001173 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001173 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001173 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001173 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_001173 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001173 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001173 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001173 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001173 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Biotin["Biotin"]
    CultureMech_001173 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_001173 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001173 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001173 --> ing24_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing24_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing25_Riboflavin["Riboflavin"]
    CultureMech_001173 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001173 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001173 --> ing27_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_D_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_001173 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001173 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_001173 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 17 G_PER_L
2 Soy peptone 3 G_PER_L
3 D-Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17634
D-glucose
4 Agar 20 G_PER_L CHEBI:2509
agar
5 NaCl
Cl.Na 58.443 g/mol
27.2 G_PER_L CHEBI:26710
sodium chloride
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
9.06 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 KCl
Cl.K 74.551 g/mol
1.28 G_PER_L CHEBI:32588
potassium chloride
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
11.88 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.02 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
11 NH4NO3
H4N.NO3 80.043 g/mol
0.0042 G_PER_L CHEBI:63038
ammonium nitrate
12 Double distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
28 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
29 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Adjust the pH to 7.3 with NaOH 4M
  2. Add to 1000 ml of medium after autoclaving and cooling to 55°C:
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.253363Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1691
2026-01-28T19:48:08.881398Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:01.897335Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001173
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.668746+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium)" to "artificial_sea_water_tryptone_soy_broth_medium_asw_tsb_medium" for consistent matching
2026-06-05T07:40:49.740092+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.538862+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.