Overview
- External ontology
- mediadive.medium:1707 (source link unavailable) — MEDIUM FOR HJ250
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1707.pdf
Composition
flowchart LR
CultureMech_001188["medium_for_hj250"]
ing0_Glucose["Glucose"]
CultureMech_001188 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_NH4Cl["NH4Cl"]
CultureMech_001188 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaHCO3["NaHCO3"]
CultureMech_001188 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_KH2PO4["KH2PO4"]
CultureMech_001188 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001188 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_NaCl["NaCl"]
CultureMech_001188 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001188 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Cysteine_HCl["Cysteine HCl"]
CultureMech_001188 --> ing7_Cysteine_HCl
ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001188 --> ing8_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
ing9_ZnCl2["ZnCl2"]
CultureMech_001188 --> ing9_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing9_ZnCl2 -.-> CHEBI_49976
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001188 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_Na2SeO3["Na2SeO3"]
CultureMech_001188 --> ing11_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing11_Na2SeO3 -.-> CHEBI_48843
ing12_H3BO3["H3BO3"]
CultureMech_001188 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001188 --> ing13_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing13_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001188 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001188 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_AlCl3["AlCl3"]
CultureMech_001188 --> ing16_AlCl3
CHEBI_30114["CHEBI:30114"]
ing16_AlCl3 -.-> CHEBI_30114
ing17_NiCl2["NiCl2"]
CultureMech_001188 --> ing17_NiCl2
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2 -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001188 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Biotin["Biotin"]
CultureMech_001188 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_001188 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001188 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl["Thiamine HCl"]
CultureMech_001188 --> ing22_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_Thiamine_HCl -.-> CHEBI_49105
ing23_Riboflavin["Riboflavin"]
CultureMech_001188 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_001188 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Pantothenate["Pantothenate"]
CultureMech_001188 --> ing25_Pantothenate
CHEBI_16454["CHEBI:16454"]
ing25_Pantothenate -.-> CHEBI_16454
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_001188 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001188 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_001188 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.53 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.61 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 | Cysteine HCl | 0.3 G_PER_L | ||
| 9 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.27 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 10 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.136 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.198 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.0017 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.0062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0033 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0013 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.13 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
AlCl3
AlCl3 133.341 g/mol
|
0.0133 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 18 |
NiCl2
Cl2Ni 129.599 g/mol
|
0.013 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0242 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.005 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.005 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Pantothenate
C9H16NO5 218.229 g/mol
|
0.005 G_PER_L |
CHEBI:16454
pantothenate
|
|
| 27 | Vitamin B12 | 0.005 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Final pH 7.5
- Prepare and boil the medium in the absence NaHCO3. Cool under a stream of N2/CO2 (80:20) and add the remaining components. Fill 10 ml of medium in 100 ml serum bottles (100 ml) under a stream of N2/CO2 (80:20) and autoclave at 121°C for 15 min. Add sterile 1.3 mM Na2S x 9 H2O and 1ml/l filter-sterilised vitamin solution (see below). Adjust the final ph to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.308636Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1707 |
| 2026-03-13T06:19:02.049993Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001188 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.843230+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR HJ250" to "medium_for_hj250" for consistent matching |
| 2026-06-05T07:41:29.097941+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.057707+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |