mmb_medium_atcc_2254

CultureMech:001205 bacterial COMPLEX SOLID_AGAR
Original name: MMB MEDIUM (ATCC 2254)

Overview

External ontology
mediadive.medium:1728 (source link unavailable) — MMB MEDIUM (ATCC 2254)
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1728.pdf

Composition

flowchart LR
    CultureMech_001205["mmb_medium_atcc_2254"]
    ing0_Peptone["Peptone"]
    CultureMech_001205 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001205 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_001205 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001205 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_Agar["Agar"]
    CultureMech_001205 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001205 --> ing5_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001205 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001205 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001205 --> ing8__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing8__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001205 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_Na_EDTA["Na-EDTA"]
    CultureMech_001205 --> ing10_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing10_Na_EDTA -.-> CHEBI_64734
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001205 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001205 --> ing12_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing12_MnSO4_x_H2O -.-> CHEBI_86364
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001205 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001205 --> ing14_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing14_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing15_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001205 --> ing15_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing15_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing16_Biotin["Biotin"]
    CultureMech_001205 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956
    ing17_Folic_acid["Folic acid"]
    CultureMech_001205 --> ing17_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing17_Folic_acid -.-> CHEBI_27470
    ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001205 --> ing18_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing19_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001205 --> ing19_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing19_Thiamine_HCl -.-> CHEBI_49105
    ing20_Riboflavin["Riboflavin"]
    CultureMech_001205 --> ing20_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavin -.-> CHEBI_17015
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001205 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001205 --> ing22_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_D_Calcium_pantothenate -.-> CHEBI_31345
    ing23_Vitamin_B12["Vitamin B12"]
    CultureMech_001205 --> ing23_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing23_Vitamin_B12 -.-> CHEBI_176843
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001205 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_Lipoic_acid["Lipoic acid"]
    CultureMech_001205 --> ing25_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing25_Lipoic_acid -.-> CHEBI_16494

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 Peptone 0.1 G_PER_L
2 Yeast extract 0.1 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.2 G_PER_L CHEBI:62946
ammonium sulfate
5 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
6 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
11 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
14 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
15 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
16 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
17 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
18 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
19 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
20 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
21 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
23 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
24 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
25 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
26 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. pH 7.0- 7.5
  2. For solid medium, add 15g of agar per liter.
  3. Glucose and vitamin solution should be filter-sterilized separately.
  4. Dissolve the nitrilotriacetic acid.
  5. Adjust pH to 7.0 with KOH (~7.3 g).
  6. Dissolve other salts separately and combine.
  7. Adjust pH to 6.8 with NaOH or H2SO4.
  8. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.366915Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1728
2026-01-28T19:48:42.320212Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:43.147915Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:02.220069Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001205
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.029648+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMB MEDIUM (ATCC 2254)" to "mmb_medium_atcc_2254" for consistent matching
2026-05-20T06:01:58.247296+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.5' -> {'min': 7.0, 'max': 7.5}
2026-06-05T07:41:35.390874+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.256990+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.