cy_s2_medium

CultureMech:001207 bacterial COMPLEX SOLID_AGAR
Original name: CY S2 MEDIUM

Overview

External ontology
mediadive.medium:172a (source link unavailable) — CY S2 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium172a.pdf

Composition

flowchart LR
    CultureMech_001207["cy_s2_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001207 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001207 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001207 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001207 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaCl["NaCl"]
    CultureMech_001207 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001207 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001207 --> ing6_Yeast_extract
    ing7_Agar["Agar"]
    CultureMech_001207 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Glucose["Glucose"]
    CultureMech_001207 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_NaHCO3["NaHCO3"]
    CultureMech_001207 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001207 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.06 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.00125 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 Yeast extract 1 G_PER_L
8 Agar 1 G_PER_L CHEBI:2509
agar
9 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
10 NaHCO3
CHO3.Na 84.006 g/mol
0.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.099 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Adjust pH to 7.0 and autoclave.
  2. After autoclaving, add from separate sterile solutions per liter medium:
  3. Aseptically fill sterile 10-ml screwcap tubes almost completely with the medium. Make stab inoculation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.403067Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 172a
2026-03-13T06:19:02.245022Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001207
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.056788+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CY S2 MEDIUM" to "cy_s2_medium" for consistent matching
2026-06-05T07:40:55.037011+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.044721+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.