Overview
- External ontology
- mediadive.medium:1750 (source link unavailable) — Enriched Artificial Seawater Medium f/2 suppl. with 50 g/l NaCl
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1750.pdf
Composition
flowchart LR
CultureMech_001224["enriched_artificial_seawater_medium_f_2_suppl_with_50_g_l_nacl"]
ing0_Artificial_Sea_Salt["Artificial Sea Salt"]
CultureMech_001224 --> ing0_Artificial_Sea_Salt
ing1_NaCl["NaCl"]
CultureMech_001224 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NaNO3["NaNO3"]
CultureMech_001224 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
CultureMech_001224 --> ing3_NaH2PO4_x_H2O
CHEBI_37585["CHEBI:37585"]
ing3_NaH2PO4_x_H2O -.-> CHEBI_37585
ing4_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_001224 --> ing4_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing4_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001224 --> ing5_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001224 --> ing6_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
ing7_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
CultureMech_001224 --> ing7_Na2_MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
ing8_D_Biotin["D-Biotin"]
CultureMech_001224 --> ing8_D_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_D_Biotin -.-> CHEBI_15956
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Artificial Sea Salt | 12.4 G_PER_L | (~ 18 mS/cm = ~ 12 psu at 25°C) | |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
50 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 4 |
NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
|
1 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 5 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
4.16 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 6 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.02196 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 7 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.8 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 8 |
Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.2 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 9 |
D-Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
Preparation
- Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
- Autoclave at 121 °C for 20 min.
- After cooling, add the following filter sterilized (0.2 µm) components to complete the medium:
Solutions
f/2 Trace Metal Mix
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl3 x 6 H2O | 3.15 G_PER_L | |
| MnCl2 x 4 H2O | 45 G_PER_L | |
| CoCl2 x 6 H2O | 1.7 G_PER_L |
Vitamin 3 Mix
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine HCl | 1 G_PER_L | |
| Vitamin B12 | 0.25 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.427323Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1750 |
| 2026-03-13T06:19:02.394498Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001224 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.218870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Enriched Artificial Seawater Medium f/2 suppl. with 50 g/l NaCl" to "enriched_artificial_seawater_medium_f_2_suppl_with_50_g_l_nacl" for consistent matching |
| 2026-06-05T07:40:59.598618+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-07T07:05:22.197477+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 3 -> 'f/2 Trace Metal Mix' @ 1 ml/l; 2 -> 'Vitamin 3 Mix' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |