pfennigs_medium_iii

Overview

External ontology

mediadive.medium:1761 (source link unavailable) — PFENNIG'S MEDIUM III

Applications

• Microbial cultivation

pH

6.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1761.pdf

Composition

flowchart LR
    CultureMech_001229["pfennigs_medium_iii"]
    ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001229 --> ing0_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing1_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001229 --> ing1_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing1_Na2S_x_9_H2O -.-> CHEBI_76209
    ing2_NaHCO3["NaHCO3"]
    CultureMech_001229 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Resazurin["Resazurin"]
    CultureMech_001229 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Ammonium_chloride["Ammonium chloride"]
    CultureMech_001229 --> ing4_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing4_Ammonium_chloride -.-> CHEBI_31206
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001229 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_KCl["KCl"]
    CultureMech_001229 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_KH2PO4["KH2PO4"]
    CultureMech_001229 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_001229 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_Ammonium_acetate["Ammonium acetate"]
    CultureMech_001229 --> ing9_Ammonium_acetate
    CHEBI_62947["CHEBI:62947"]
    ing9_Ammonium_acetate -.-> CHEBI_62947
    ing10_Magnesium_acetate["Magnesium acetate"]
    CultureMech_001229 --> ing10_Magnesium_acetate
    CHEBI_62964["CHEBI:62964"]
    ing10_Magnesium_acetate -.-> CHEBI_62964
    ing11_Na2_EDTA["Na2-EDTA"]
    CultureMech_001229 --> ing11_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing11_Na2_EDTA -.-> CHEBI_64734
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001229 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001229 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_001229 --> ing14_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing14_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing15_ZnCl2["ZnCl2"]
    CultureMech_001229 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001229 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001229 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_H3BO3["H3BO3"]
    CultureMech_001229 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001229 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (20)

Preparation

1. Adjust pH to 6.0.
2. Mix solution D, C and E in the cotton-stoppered Erlenmeyer flask. Bubble with CO2 in an ice bath under sterile conditions (5-10 min).
3. Fill 50 ml of the mixed solution (C,D,E) into each 100 ml bottle of solution A (for a total volume of approx. 96 ml).
4. Before using, add 2,6 ml solution B (Na2S x 9 H2O), 0.1 ml solution F (Vitamin B12) and 1 ml of solution G (2,5% Ammonium-acetate and Magnesium-acetate) to each 100ml bottle.
5. Adjust the pH with filter-sterilised 1M Na2CO3 to 7.1-7.3.
6. Fill in sterile, N2 gassed screw-cap tubes under N2 gas.
7. For the growth of DSM 11081 feed the culture with neutralized sulfide feeding solution regularly (approx. every 3 days). 0,5-1 ml/ 100 mL
8. Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave at 121°C for 15 min.
9. Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave.
10. Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle.
11. Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath (10-15 min).
12. Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle.
13. Filter sterilized
14. Autoclave at 121°C for 15 min.
15. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Target organisms

No target organisms are recorded.

Provenance