anb_aerobic

CultureMech:001235 bacterial COMPLEX LIQUID
Original name: ANB aerobic

Overview

External ontology
mediadive.medium:1778a (source link unavailable) — ANB aerobic
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001235["anb_aerobic"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_001235 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_001235 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_001235 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001235 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4["MgSO4"]
    CultureMech_001235 --> ing4_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing4_MgSO4 -.-> CHEBI_32599
    ing5_NH4Cl["NH4Cl"]
    CultureMech_001235 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Melezitose["Melezitose"]
    CultureMech_001235 --> ing6_Melezitose
    CHEBI_6731["CHEBI:6731"]
    ing6_Melezitose -.-> CHEBI_6731
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001235 --> ing7_Yeast_extract
    ing8_EDTA["EDTA"]
    CultureMech_001235 --> ing8_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing8_EDTA -.-> CHEBI_4735
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001235 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_ZnSO4["ZnSO4"]
    CultureMech_001235 --> ing10_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing10_ZnSO4 -.-> CHEBI_35176
    ing11_MnCl2["MnCl2"]
    CultureMech_001235 --> ing11_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing11_MnCl2 -.-> CHEBI_63041
    ing12_H3BO3["H3BO3"]
    CultureMech_001235 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001235 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_001235 --> ing14_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing14_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001235 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001235 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Thiamine["Thiamine"]
    CultureMech_001235 --> ing17_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing17_Thiamine -.-> CHEBI_18385
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001235 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Biotin["Biotin"]
    CultureMech_001235 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_PABA["PABA"]
    CultureMech_001235 --> ing20_PABA
    CHEBI_30753["CHEBI:30753"]
    ing20_PABA -.-> CHEBI_30753
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001235 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Pyridoxine["Pyridoxine"]
    CultureMech_001235 --> ing22_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing22_Pyridoxine -.-> CHEBI_16709
    ing23_NaOH["NaOH"]
    CultureMech_001235 --> ing23_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing23_NaOH -.-> CHEBI_32145
    ing24_Folic_acid["Folic acid"]
    CultureMech_001235 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Riboflavin["Riboflavin"]
    CultureMech_001235 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_B12["B12"]
    CultureMech_001235 --> ing26_B12

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
15 G_PER_L CHEBI:29377
sodium carbonate
2 NaHCO3
CHO3.Na 84.006 g/mol
20 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 NaCl
Cl.Na 58.443 g/mol
3 G_PER_L CHEBI:26710
sodium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgSO4
Mg.O4S 120.368 g/mol
0.12037 G_PER_L CHEBI:32599
magnesium sulfate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.21396 G_PER_L CHEBI:31206
ammonium chloride
7 Melezitose
C18H32O16 504.438 g/mol
2 G_PER_L CHEBI:6731
melezitose
8 Yeast extract 0.02 G_PER_L
9 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
12 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
13 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.01 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 Thiamine
C12H17N4OS 265.362 g/mol
0.05 G_PER_L CHEBI:18385
thiamine(1+)
19 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
21 PABA
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
23 Pyridoxine
C8H11NO3 169.18 g/mol
0.1 G_PER_L CHEBI:16709
pyridoxine
24 NaOH
HO.Na 39.997 g/mol
100 G_PER_L CHEBI:32145
sodium hydroxide
25 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
27 B12 0.005 G_PER_L

Preparation

  1. mix compounds.
  2. check pH (should be 10)
  3. Fill 100 mL each into 200 mL serum-flasks.
  4. Autoclave at 121 °C for 20 min.
  5. After autoclaving, add the following sterile solutions:
  6. Mix well and aliquot into the desired volumes and flasks or tubes (e.g., 20 ml in 100 ml flasks).
  7. Adjust pH to 3.0-4.0 with HCl.
  8. Autoclave at 121 °C for 15 min.
  9. Note: autoclave in closed bottles
  10. Sterilize by filtration.
  11. Mix the four solutions in the ratio 1:1:1:1.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.452061Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1778a
2026-02-06T05:10:44.277921Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:02.461828Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001235
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.290138+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANB aerobic" to "anb_aerobic" for consistent matching
2026-06-05T07:40:49.188065+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.344755+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:01:32.232927+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).