bbm_v

CultureMech:001244 bacterial DEFINED LIQUID
Original name: BBM + V

Overview

External ontology
mediadive.medium:1802 (source link unavailable) — BBM + V
Applications
  • Microbial cultivation
pH
6.4
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001244["bbm_v"]
    ing0_NaNO3["NaNO3"]
    CultureMech_001244 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001244 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001244 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001244 --> ing3_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_001244 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NaCl["NaCl"]
    CultureMech_001244 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Na_EDTA["Na-EDTA"]
    CultureMech_001244 --> ing6_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na_EDTA -.-> CHEBI_64734
    ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001244 --> ing7_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001244 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_ZnCl2["ZnCl2"]
    CultureMech_001244 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001244 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001244 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_Thiamine["Thiamine"]
    CultureMech_001244 --> ing12_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing12_Thiamine -.-> CHEBI_18385
    ing13_Biotine["Biotine"]
    CultureMech_001244 --> ing13_Biotine
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotine -.-> CHEBI_15956
    ing14_Vitamine_B12["Vitamine B12"]
    CultureMech_001244 --> ing14_Vitamine_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamine_B12 -.-> CHEBI_176843

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
0.833 G_PER_L CHEBI:63005
sodium nitrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
6.94 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
17.24 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 KH2PO4
H2O4P.K 136.084 g/mol
23.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
7 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.75 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
30.97 G_PER_L CHEBI:86254
iron trichloride hexahydrate
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
10.25 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
4.16 G_PER_L CHEBI:49976
zinc dichloride
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.33 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
20 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 Thiamine
C12H17N4OS 265.362 g/mol
10 G_PER_L CHEBI:18385
thiamine(1+)
14 Biotine
C10H16N2O3S 244.316 g/mol
10 G_PER_L CHEBI:15956
biotin
15 Vitamine B12 0.75 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with Milli Q water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling, add the following filter sterilized components:
  4. To 900 ml of Milli Q water, add 750 mg of Na2EDTA, and dissolve fully.
  5. Add the following salts in the order and amounts indicated:
  6. Adjust pH to 6.4.
  7. Make up to <span class='amount' data-volume='1000'>1000</span> ml with Milli Q water.
  8. Filtrate sterile (pore size 0.22 µm) and aliquot.
  9. Make up to <span class='amount' data-volume='30'>30</span> ml with Milli Q water.
  10. Filtrate sterile (pore size 0.22 µm) and aliquot.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.478201Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1802
2026-03-13T06:19:02.533189Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001244
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.368726+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BBM + V" to "bbm_v" for consistent matching
2026-06-05T07:40:50.669278+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.836827+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.