mda (CultureMech:001249)

Overview

External ontology

mediadive.medium:1816 (source link unavailable) — MDA

Applications

Microbial cultivation

Notes

MediaDive DSMZ Medium 1816 records MDA as an official DSMZ medium whose Main sol. 1816 formula is a 500 ml source batch: 18 g malt extract, 5 g Bacto peptone, 10 g Desicatted Ox-bile, 5 g Tween 40, 1 ml glycerol, 1 ml olive oil, 0.25 g (-)-Chloramphenicol, 7.5 g agar, and 500 ml distilled water. Concentrations are normalized here to one liter. MediaDive reports final pH as n.d.

Composition

flowchart LR
    CultureMech_001249["mda"]
    ing0_Malt_extract["Malt extract"]
    CultureMech_001249 --> ing0_Malt_extract
    ing1_Bacto_peptone["Bacto peptone"]
    CultureMech_001249 --> ing1_Bacto_peptone
    ing2_Desicatted_Ox_bile["Desicatted Ox-bile"]
    CultureMech_001249 --> ing2_Desicatted_Ox_bile
    ing3_Tween_40["Tween 40"]
    CultureMech_001249 --> ing3_Tween_40
    CHEBI_53423["CHEBI:53423"]
    ing3_Tween_40 -.-> CHEBI_53423
    ing4_Glycerol["Glycerol"]
    CultureMech_001249 --> ing4_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing4_Glycerol -.-> CHEBI_17754
    ing5_Olive_oil["Olive oil"]
    CultureMech_001249 --> ing5_Olive_oil
    ing6_____Chloramphenicol["(-)-Chloramphenicol"]
    CultureMech_001249 --> ing6_____Chloramphenicol
    CHEBI_17698["CHEBI:17698"]
    ing6_____Chloramphenicol -.-> CHEBI_17698
    ing7_Agar["Agar"]
    CultureMech_001249 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Distilled_water["Distilled water"]
    CultureMech_001249 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Malt extract	36 G_PER_L	FOODON:03301056 malt extract	MediaDive DSMZ Medium 1816 lists 36 g/L Malt extract.
2	Bacto peptone	10 G_PER_L	MICRO:0000178 (source link unavailable) Bacto peptone	MediaDive DSMZ Medium 1816 lists 10 g/L Bacto peptone.
3	Desicatted Ox-bile	20 G_PER_L		MediaDive DSMZ Medium 1816 lists 20 g/L Desicatted Ox-bile.
4	Tween 40 (C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C22H42O6 578.784 g/mol	10 G_PER_L	CHEBI:53423 polysorbate 40	MediaDive DSMZ Medium 1816 lists 10 g/L Tween 40.
5	Glycerol C3H8O3 92.094 g/mol	2 ML_PER_L	CHEBI:17754 glycerol	MediaDive DSMZ Medium 1816 lists 2 ml/L Glycerol.
6	Olive oil	2 ML_PER_L		MediaDive DSMZ Medium 1816 lists 2 ml/L Olive oil.
7	(-)-Chloramphenicol C11H12Cl2N2O5 323.132 g/mol	0.5 G_PER_L	CHEBI:17698 chloramphenicol	MediaDive DSMZ Medium 1816 lists 0.5 g/L (-)-Chloramphenicol.
8	Agar	15 G_PER_L	CHEBI:2509 agar	MediaDive DSMZ Medium 1816 lists 15 g/L Agar.
9	Distilled water H2O 18.015 g/mol	1000 ML_PER_L	CHEBI:15377 water	MediaDive DSMZ Medium 1816 lists 1000 ml/L Distilled water.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.488675Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1816
2026-03-13T06:19:02.554068Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001249
2026-03-17T06:30:20.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.392691+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MDA" to "mda" for consistent matching
2026-06-05T07:41:13.475556+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00	repair_mediadive_1816_mda_score15.py	RESOLVED_MEDIADIVE_1816_MDA_SCORE15	Corrected the MediaDive 500 ml source batch to per-liter concentrations, restored Distilled water as a measured ml/L component, preserved source names for the Desicatted Ox-bile and olive-oil mixture rows, and grounded exact malt extract, Bacto peptone, Tween 40, glycerol, chloramphenicol, agar, and water components.