Overview
- External ontology
- mediadive.medium:1820 (source link unavailable) — Med 333
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001253["med_333"]
ing0_Glucose["Glucose"]
CultureMech_001253 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Starch["Starch"]
CultureMech_001253 --> ing1_Starch
CHEBI_28017["CHEBI:28017"]
ing1_Starch -.-> CHEBI_28017
ing2_Yeast_extract["Yeast extract"]
CultureMech_001253 --> ing2_Yeast_extract
ing3_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_001253 --> ing3_Proteose_peptone_no__3
ing4_CaCO3["CaCO3"]
CultureMech_001253 --> ing4_CaCO3
CHEBI_3311["CHEBI:3311"]
ing4_CaCO3 -.-> CHEBI_3311
ing5_NH4NO3["NH4NO3"]
CultureMech_001253 --> ing5_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing5_NH4NO3 -.-> CHEBI_63038
ing6_Agar["Agar"]
CultureMech_001253 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Starch | 10 G_PER_L |
CHEBI:28017
starch
|
|
| 3 | Yeast extract | 3 G_PER_L | ||
| 4 | Proteose peptone no. 3 | 3 G_PER_L | ||
| 5 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 6 |
NH4NO3
H4N.NO3 80.043 g/mol
|
3 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.2.
- Autoclave at 121 °C for 20 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.493013Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1820 |
| 2026-01-28T19:48:18.835649Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:02.571026Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001253 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.410163+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Med 333" to "med_333" for consistent matching |
| 2026-06-05T07:41:13.529632+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.086031+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |