mdm

CultureMech:001254 bacterial DEFINED LIQUID
Original name: MDM

Overview

External ontology
mediadive.medium:1825 (source link unavailable) — MDM
Applications
  • Microbial cultivation
pH range
{'min': 6.0, 'max': 7.0}
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001254["mdm"]
    ing0_Na2HPO4["Na2HPO4"]
    CultureMech_001254 --> ing0_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4 -.-> CHEBI_34683
    ing1_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001254 --> ing1_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001254 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NaCl["NaCl"]
    CultureMech_001254 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001254 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001254 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001254 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001254 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001254 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_NaHSeO3["NaHSeO3"]
    CultureMech_001254 --> ing9_NaHSeO3
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001254 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_D_Biotin["D-Biotin"]
    CultureMech_001254 --> ing11_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_D_Biotin -.-> CHEBI_15956
    ing12_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001254 --> ing12_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing12_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing13_Pyridoxine["Pyridoxine"]
    CultureMech_001254 --> ing13_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing13_Pyridoxine -.-> CHEBI_16709
    ing14_alpha_D_Glucose["alpha-D-Glucose"]
    CultureMech_001254 --> ing14_alpha_D_Glucose
    CHEBI_17925["CHEBI:17925"]
    ing14_alpha_D_Glucose -.-> CHEBI_17925
    ing15_L_Cysteine["L-Cysteine"]
    CultureMech_001254 --> ing15_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing15_L_Cysteine -.-> CHEBI_17561
    ing16_L_Tryptophan["L-Tryptophan"]
    CultureMech_001254 --> ing16_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing16_L_Tryptophan -.-> CHEBI_16828
    ing17_L_Leucine["L-Leucine"]
    CultureMech_001254 --> ing17_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing17_L_Leucine -.-> CHEBI_15603
    ing18_L_Valine["L-Valine"]
    CultureMech_001254 --> ing18_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing18_L_Valine -.-> CHEBI_16414
    ing19_L_Proline["L-Proline"]
    CultureMech_001254 --> ing19_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing19_L_Proline -.-> CHEBI_17203
    ing20_L_Isoleucine["L-Isoleucine"]
    CultureMech_001254 --> ing20_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing20_L_Isoleucine -.-> CHEBI_17191
    ing21_L_Methionine["L-Methionine"]
    CultureMech_001254 --> ing21_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing21_L_Methionine -.-> CHEBI_16643

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Na2HPO4
HO4P.2Na 141.958 g/mol
50 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
20 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
9 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NaCl
Cl.Na 58.443 g/mol
9 G_PER_L CHEBI:26710
sodium chloride
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
4 G_PER_L CHEBI:62946
ammonium sulfate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.1 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 NaHSeO3 0.015 G_PER_L (almost no effect on growth in CDMM, more important in MDM)
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
12 D-Biotin
C10H16N2O3S 244.316 g/mol
0.06 G_PER_L CHEBI:15956
biotin
13 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.2 G_PER_L CHEBI:31345
Calcium pantothenate
14 Pyridoxine
C8H11NO3 169.18 g/mol
0.2 G_PER_L CHEBI:16709
pyridoxine
15 alpha-D-Glucose
C6H12O6 180.156 g/mol
200 G_PER_L CHEBI:17925
alpha-D-glucose
 (not required but growth enhancing)
16 L-Cysteine
C3H7NO2S 121.161 g/mol
10 G_PER_L CHEBI:17561
L-cysteine
17 L-Tryptophan
C11H12N2O2 204.229 g/mol
0.5 G_PER_L CHEBI:16828
L-tryptophan
18 L-Leucine
C6H13NO2 131.175 g/mol
2 G_PER_L CHEBI:15603
L-leucine
19 L-Valine
C5H11NO2 117.148 g/mol
1.5 G_PER_L CHEBI:16414
L-valine
20 L-Proline
C5H9NO2 115.132 g/mol
10 G_PER_L CHEBI:17203
L-proline
21 L-Isoleucine
C6H13NO2 131.175 g/mol
1.5 G_PER_L CHEBI:17191
L-isoleucine
22 L-Methionine
C5H11NO2S 149.215 g/mol
1 G_PER_L CHEBI:16643
L-methionine

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with sterile distilled water.
  2. anaerobize with nitrogen, can be stored anaerobically for a limited time
  3. autoclave
  4. steril filtration
  5. steril filtration, store in aliquots at -20°C
  6. steril filtration, store aliquots at -20°C
  7. steril filtration
  8. Adjust pH to 6.0-7.0.
  9. Filtrate.
  10. Store at -20°C

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.494886Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1825
2026-02-06T05:10:51.266245Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:02.580409Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001254
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.420158+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MDM" to "mdm" for consistent matching
2026-05-20T06:01:25.885735+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6-7' -> {'min': 6.0, 'max': 7.0}
2026-06-05T07:41:13.485663+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:01.070153+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.