saccharolobus_mt_4_medium

CultureMech:001258 archaea COMPLEX LIQUID
Original name: SACCHAROLOBUS (MT-4) MEDIUM

Overview

External ontology
mediadive.medium:182a (source link unavailable) — SACCHAROLOBUS (MT-4) MEDIUM
Applications
  • Microbial cultivation
pH
3.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium182a.pdf

Composition

flowchart LR
    CultureMech_001258["saccharolobus_mt_4_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001258 --> ing0_Yeast_extract
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001258 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001258 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001258 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001258 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_HCl["HCl"]
    CultureMech_001258 --> ing5_HCl
    CHEBI_17883["CHEBI:17883"]
    ing5_HCl -.-> CHEBI_17883
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001258 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_ZnCl2["ZnCl2"]
    CultureMech_001258 --> ing7_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing7_ZnCl2 -.-> CHEBI_49976
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001258 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_001258 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001258 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001258 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001258 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001258 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2 G_PER_L
2 KH2PO4
H2O4P.K 136.084 g/mol
3.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2.5 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
7 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
8 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH of the medium at room temperature to 3.5 with 10 N H2SO4 prior to autoclaving.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.411418Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 182a
2026-03-13T06:19:02.610857Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001258
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.454277+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SACCHAROLOBUS (MT-4) MEDIUM" to "saccharolobus_mt_4_medium" for consistent matching
2026-06-05T07:41:45.003600+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.217523+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.