cyanobacteria_medium_1_2_swes_brackish_water_medium

CultureMech:001261 bacterial COMPLEX LIQUID
Original name: Cyanobacteria Medium 1/2 SWES - Brackish Water Medium

Overview

External ontology
mediadive.medium:1831a (source link unavailable) — Cyanobacteria Medium 1/2 SWES - Brackish Water Medium
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001261["cyanobacteria_medium_1_2_swes_brackish_water_medium"]
    ing0_Natural_sea_water["Natural sea water"]
    CultureMech_001261 --> ing0_Natural_sea_water
    ing1_KNO3["KNO3"]
    CultureMech_001261 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001261 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001261 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Vitamin_B12["Vitamin B12"]
    CultureMech_001261 --> ing4_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing4_Vitamin_B12 -.-> CHEBI_176843
    ing5_Fe_SO4_x_7_H2O["Fe SO4 x 7 H2O"]
    CultureMech_001261 --> ing5_Fe_SO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_Fe_SO4_x_7_H2O -.-> CHEBI_75836
    ing6_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
    CultureMech_001261 --> ing6_Na_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing6_Na_EDTA_x_2_H2O -.-> CHEBI_64758
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001261 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_MnSO4_x_1_H2O["MnSO4 x 1 H2O"]
    CultureMech_001261 --> ing8_MnSO4_x_1_H2O
    CHEBI_86360["CHEBI:86360"]
    ing8_MnSO4_x_1_H2O -.-> CHEBI_86360
    ing9_H3BO3["H3BO3"]
    CultureMech_001261 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001261 --> ing10_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing10_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing11_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_001261 --> ing11_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001261 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_Garden_soil["Garden soil"]
    CultureMech_001261 --> ing13_Garden_soil

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Natural sea water 455 G_PER_L (Helgolandwasser)
2 KNO3
K.NO3 101.102 g/mol
10.5 G_PER_L CHEBI:63043
potassium nitrate
3 K2HPO4
HO4P.2K 174.174 g/mol
4.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Vitamin B12 0.25 G_PER_L CHEBI:176843
vitamin B12
6 Fe SO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
70 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
10 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 MnSO4 x 1 H2O
Mn.O4S 151.001 g/mol
2 G_PER_L CHEBI:86360
manganese(II) sulfate
10 H3BO3
H3BO3 61.833 g/mol
5 G_PER_L CHEBI:33118
boric acid
11 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.2 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
12 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
5 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
20 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
14 Garden soil 300 G_PER_L

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling, add the following filter sterilized components:
  4. Make up to <span class='amount' data-volume='1000'>1000</span> ml with Milli Q water.
  5. Prepare aliquots and store them at -20 °C.
  6. Fill 300ml garden soil in a 1 l laboratory flask and fill up with H2Odist to 700 ml (The soil should not contain too much humus and not too much clay – avoid soil contaminated with fertilizers, herbicides or fungicides)
  7. Shake vigorously
  8. Autoclave for 1 h at 121 °C and let cool down
  9. After 24 h autoclave for a second time (for 1 h at 121 °C)
  10. Remove bigger particles and debris by centrifugation
  11. Filter through Whatmanfilter 595 (1/2, 320 mm) to remove residual particles
  12. Filter sterilize (PES membrane, pore size 0,22 µm)
  13. Store at 4°C – 10°C

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.510801Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1831a
2026-03-13T06:19:02.632202Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001261
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.477232+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cyanobacteria Medium 1/2 SWES - Brackish Water Medium" to "cyanobacteria_medium_1_2_swes_brackish_water_medium" for consistent matching
2026-06-05T07:40:55.046471+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.663719+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:55.047863+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.