halorutilales_haloarchaeon_medium

CultureMech:001265 archaea COMPLEX LIQUID
Original name: Halorutilales haloarchaeon medium

Overview

External ontology
mediadive.medium:1840 (source link unavailable) — Halorutilales haloarchaeon medium
Applications
  • Microbial cultivation
pH
6.8
High-metal flag
True
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001265["halorutilales_haloarchaeon_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001265 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001265 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KCl["KCl"]
    CultureMech_001265 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001265 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001265 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Na_thiosulfate["Na-thiosulfate"]
    CultureMech_001265 --> ing5_Na_thiosulfate
    CHEBI_132112["CHEBI:132112"]
    ing5_Na_thiosulfate -.-> CHEBI_132112
    ing6_NaHCO3["NaHCO3"]
    CultureMech_001265 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_EDTA["EDTA"]
    CultureMech_001265 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001265 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4["ZnSO4"]
    CultureMech_001265 --> ing9_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing9_ZnSO4 -.-> CHEBI_35176
    ing10_MnCl2["MnCl2"]
    CultureMech_001265 --> ing10_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing10_MnCl2 -.-> CHEBI_63041
    ing11_H3BO3["H3BO3"]
    CultureMech_001265 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001265 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_001265 --> ing13_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing13_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001265 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001265 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_Thiamine["Thiamine"]
    CultureMech_001265 --> ing16_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing16_Thiamine -.-> CHEBI_18385
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001265 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Biotin["Biotin"]
    CultureMech_001265 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_PABA["PABA"]
    CultureMech_001265 --> ing19_PABA
    CHEBI_30753["CHEBI:30753"]
    ing19_PABA -.-> CHEBI_30753
    ing20_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001265 --> ing20_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_acid -.-> CHEBI_15940
    ing21_Pyridoxine["Pyridoxine"]
    CultureMech_001265 --> ing21_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing21_Pyridoxine -.-> CHEBI_16709
    ing22_NaOH["NaOH"]
    CultureMech_001265 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Folic_acid["Folic acid"]
    CultureMech_001265 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Riboflavin["Riboflavin"]
    CultureMech_001265 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_B12["B12"]
    CultureMech_001265 --> ing25_B12
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001265 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001265 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
4 NH4Cl
Cl.H4N 53.492 g/mol
0.2 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0197181 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Na-thiosulfate
2Na.O3S2 158.111 g/mol
3.1622 G_PER_L CHEBI:132112
sodium thiosulfate
7 NaHCO3
CHO3.Na 84.006 g/mol
0.84 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
11 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.01 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 Thiamine
C12H17N4OS 265.362 g/mol
0.05 G_PER_L CHEBI:18385
thiamine(1+)
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
20 PABA
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
22 Pyridoxine
C8H11NO3 169.18 g/mol
0.1 G_PER_L CHEBI:16709
pyridoxine
23 NaOH
HO.Na 39.997 g/mol
100.04 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 100.0, 0.04]
24 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
26 B12 0.005 G_PER_L
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.026 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.03 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Adjust pH to 6.8 with 0.5 M KH2PO4.
  2. Dispense into serum bottles (30 ml medium in 100 ml serum bottles, OR 15 ml in 50 serum bottles).
  3. add 20% CO2 to gas phase (in serum bottles)
  4. Autoclave at 121 °C for 20 min.
  5. Before inoculation add from sterile stock solutions per liter:
  6. Adjust pH to 3.0-4.0 with HCl.
  7. Autoclave at 121 °C for 15 min.
  8. Note: autoclave in closed bottles
  9. Sterilize by filtration.
  10. Mix the four solutions in the ratio 1:1:1:1.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.523943Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1840
2026-02-06T05:10:12.238885Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:37.993426Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:02.668660Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001265
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.515797+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halorutilales haloarchaeon medium" to "halorutilales_haloarchaeon_medium" for consistent matching
2026-06-05T07:41:08.203777+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.661894+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.