nitrosotalea_aoa_fresh_water_medium_fw

Overview

External ontology

mediadive.medium:1844 (source link unavailable) — Nitrosotalea (AOA) Fresh Water Medium (FW)

Applications

• Microbial cultivation

pH

5.1

Notes

Source: DSMZ

Composition

flowchart LR
    CultureMech_001267["nitrosotalea_aoa_fresh_water_medium_fw"]
    ing0_MES_Hydrat["MES Hydrat"]
    CultureMech_001267 --> ing0_MES_Hydrat
    ing1_NaCl["NaCl"]
    CultureMech_001267 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001267 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001267 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_001267 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_KCl["KCl"]
    CultureMech_001267 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NH4Cl["NH4Cl"]
    CultureMech_001267 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Double_distilled_water["Double distilled water"]
    CultureMech_001267 --> ing7_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Double_distilled_water -.-> CHEBI_15377
    ing8_NaHCO3["NaHCO3"]
    CultureMech_001267 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_HCl["HCl"]
    CultureMech_001267 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_H3BO3["H3BO3"]
    CultureMech_001267 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001267 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001267 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001267 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001267 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001267 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001267 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_001267 --> ing17_FeNa_EDTA

Ingredients (18)

Preparation

1. Crucial: prepare all solutions in either clean glassware (incubated in 0.1 % HCl and washed with double distilled water) or GREINER PS container (201172). Do not use container with rubber stoppers or septum.
2. Add 100 mL of 10x FW basal salt solution to 800 mL sterile double distilled water just before use.
3. Add per litre of 1x FW basalt solution: 0.5 mL NH4Cl (1 M), 2 mL NaHCO3 (1 M), 1 mL FeNaEDTA (7.5 mM), 1 mL trace elements solution, 1.95 g MES Hydrat.
4. Adjust pH to the specific pH for each strain.
5. Make up to <span class='amount' data-volume='1000'>1000</span> ml with double distilled water.
6. Filtrate with 0.2 µm Nalgene® bottle-top sterile filter units (e.g. ThermoFischer 597-4520) in a clean and sterile glass bottle.
7. Aliquote medium in GREINER PS containers. Final medium can be stored at 4 °C in the dark for weeks.
8. Prepare FW basal salt solution (10x) in double distilled water.
9. Autoclave at 121 °C for 20 min.
10. Cool down to room temperature.
11. 10x FW basal salt solution can be stored at 4 °C for months.
12. Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
13. Filtrate (0.2 µm).
14. Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 µm) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 °C. Avoid high headspace to liquid ratios to ensure stable concentration.
15. This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.
16. Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.

Target organisms

No target organisms are recorded.

Provenance