nitrososphaera_gargensis_medium

CultureMech:001268 archaea DEFINED LIQUID
Original name: Nitrososphaera gargensis medium

Overview

External ontology
mediadive.medium:1845 (source link unavailable) — Nitrososphaera gargensis medium
Applications
  • Microbial cultivation
pH
8.5
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001268["nitrososphaera_gargensis_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001268 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_KCl["KCl"]
    CultureMech_001268 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Mg_SO4__x_7_H2O["Mg(SO4) x 7 H2O"]
    CultureMech_001268 --> ing2_Mg_SO4__x_7_H2O
    ing3_NaCl["NaCl"]
    CultureMech_001268 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_HCl["HCl"]
    CultureMech_001268 --> ing4_HCl
    CHEBI_17883["CHEBI:17883"]
    ing4_HCl -.-> CHEBI_17883
    ing5_H3BO3["H3BO3"]
    CultureMech_001268 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001268 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001268 --> ing7_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001268 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001268 --> ing9_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001268 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001268 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_Double_distilled_water["Double distilled water"]
    CultureMech_001268 --> ing12_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing12_Double_distilled_water -.-> CHEBI_15377
    ing13_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_001268 --> ing13_FeNa_EDTA
    ing14_NaOH["NaOH"]
    CultureMech_001268 --> ing14_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing14_NaOH -.-> CHEBI_32145
    ing15_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001268 --> ing15_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing15_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001268 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_NH4Cl["NH4Cl"]
    CultureMech_001268 --> ing17_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing17_NH4Cl -.-> CHEBI_31206

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 KCl
Cl.K 74.551 g/mol
1.5 G_PER_L CHEBI:32588
potassium chloride
3 Mg(SO4) x 7 H2O 7 G_PER_L
4 NaCl
Cl.Na 58.443 g/mol
11.68 G_PER_L CHEBI:26710
sodium chloride
5 HCl
HCl 36.461 g/mol
3.646 G_PER_L CHEBI:17883
hydrogen chloride
6 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
10 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 Double distilled water
H2O 18.015 g/mol
2050.0 G_PER_L CHEBI:15377
water
 [Merged 3 duplicates: 1000.0, 1000.0, 50.0]
14 FeNa-EDTA 2.753 G_PER_L
15 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
16 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 NH4Cl
Cl.H4N 53.492 g/mol
53.4 G_PER_L CHEBI:31206
ammonium chloride

Preparation

  1. Add 50 mL of 20x Basal salt stock solution and 4 g CaCO3 to 950 mL sterile double distilled water and mix all just before use.
  2. Autoclave at 121 °C for 20 min.
  3. Cool down to room temperature.
  4. Add aseptic per liter of 1x basalt salt solution: 1 mL FeNaEDTA (7.5 mM), 1 mL trace elements solution, 1 mL Selenite-tungstate solution, 0.5 mL NH4Cl solution.
  5. Adjust pH to 8.5 with NaOH.
  6. Prepare with double distilled water.
  7. Autoclave at 121 °C for 20 min.
  8. Cool down to room temperature.
  9. Store in the dark at 4 °C.
  10. This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.
  11. Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.
  12. Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
  13. Filtrate (0.2 µm).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.540240Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1845
2026-03-13T03:08:45.958356Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:02.708515Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001268
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.557081+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nitrososphaera gargensis medium" to "nitrososphaera_gargensis_medium" for consistent matching
2026-06-05T07:41:39.882938+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.624783+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.