cultivation_medium_for_sf1ep_cells_for_treponema_pallidum (CultureMech:001271)

Overview

External ontology

mediadive.medium:1850 (source link unavailable) — Cultivation Medium for Sf1Ep cells for Treponema pallidum

Applications

Microbial cultivation

Notes

DSMZ Medium 1850 records a Sf1Ep cottontail rabbit epithelial cell medium prepared from Eagle's MEM, MEM Non-Essential Amino Acids, L-glutamine, sodium pyruvate, and heat-inactivated fetal bovine serum, then filter-sterilized and stored at 4 C.

Composition

flowchart LR
    CultureMech_001271["cultivation_medium_for_sf1ep_cells_for_treponema_pallidum"]
    ing0_Eagle_s_MEM["Eagle's MEM"]
    CultureMech_001271 --> ing0_Eagle_s_MEM
    ing1_MEM_Non_Essential_Amino_Acids["MEM Non-Essential Amino Acids"]
    CultureMech_001271 --> ing1_MEM_Non_Essential_Amino_Acids
    ing2_L_glutamine_solution["L-glutamine solution"]
    CultureMech_001271 --> ing2_L_glutamine_solution
    CHEBI_18050["CHEBI:18050"]
    ing2_L_glutamine_solution -.-> CHEBI_18050
    ing3_Sodium_pyruvate_solution["Sodium pyruvate solution"]
    CultureMech_001271 --> ing3_Sodium_pyruvate_solution
    CHEBI_50144["CHEBI:50144"]
    ing3_Sodium_pyruvate_solution -.-> CHEBI_50144
    ing4_Fetal_bovine_serum__heat_inactivated["Fetal bovine serum, heat inactivated"]
    CultureMech_001271 --> ing4_Fetal_bovine_serum__heat_inactivated

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Eagle's MEM Sigma M4655	884.956 ML_PER_L		DSMZ Medium 1850 lists 500 ml Eagle's MEM (Sigma M4655) in a 565 ml Sf1Ep medium batch.
2	MEM Non-Essential Amino Acids Gibco 11140-050	8.84956 ML_PER_L		DSMZ Medium 1850 lists 5 ml MEM Non-Essential Amino Acids (Gibco 11140-050) in a 565 ml Sf1Ep medium batch.
3	L-glutamine solution C5H10N2O3 146.146 g/mol Sigma G7513	8.84956 ML_PER_L	CHEBI:18050 L-glutamine	DSMZ Medium 1850 lists 5 ml L-glutamine (Sigma G7513) in a 565 ml Sf1Ep medium batch; the stock concentration is not disclosed.
4	Sodium pyruvate solution C3H3O3.Na 110.044 g/mol Sigma S8636	8.84956 ML_PER_L	CHEBI:50144 sodium pyruvate	DSMZ Medium 1850 lists 5 ml sodium pyruvate (Sigma S8636) in a 565 ml Sf1Ep medium batch; the stock concentration is not disclosed.
5	Fetal bovine serum, heat inactivated	88.4956 ML_PER_L		DSMZ Medium 1850 lists 50 ml heat-inactivated fetal bovine serum in a 565 ml Sf1Ep medium batch.

Preparation

Combine Eagle's MEM, MEM Non-Essential Amino Acids, L-glutamine, sodium pyruvate, and heat-inactivated fetal bovine serum at the DSMZ Medium 1850 Sf1Ep medium ratios.
Filter sterilize the Sf1Ep medium.
Store the filter-sterilized Sf1Ep medium at 4 C.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.549179Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1850
2026-02-03T11:26:48.362345	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:02.722853Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001271
2026-03-17T19:45:14.572756+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Cultivation Medium for Sf1Ep cells for Treponema pallidum" to "cultivation_medium_for_sf1ep_cells_for_treponema_pallidum" for consistent matching
2026-08-06T07:56:36.091743+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-09-08T00:00:00-07:00	repair_dsmz_1850_score35.py	RESOLVED_DSMZ_1850_SCORE35_GRAPH	MediaDive's structured DSMZ Medium 1850 main solution is empty, but its DSMZ description explicitly lists a 565 ml Sf1Ep medium batch: 500 ml Eagle's MEM, 5 ml MEM Non-Essential Amino Acids, 5 ml L-glutamine, 5 ml sodium pyruvate, and 50 ml heat-inactivated fetal bovine serum.