pilobolus_medium

CultureMech:001284 bacterial COMPLEX SOLID_AGAR
Original name: PILOBOLUS MEDIUM

Overview

External ontology
mediadive.medium:192 (source link unavailable) — PILOBOLUS MEDIUM
Applications
  • Microbial cultivation
pH
8.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium192.pdf

Composition

flowchart LR
    CultureMech_001284["pilobolus_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001284 --> ing0_Yeast_extract
    ing1_Na_acetate["Na-acetate"]
    CultureMech_001284 --> ing1_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing1_Na_acetate -.-> CHEBI_32954
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001284 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001284 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001284 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001284 --> ing5_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing5_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing6_Haemin["Haemin"]
    CultureMech_001284 --> ing6_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing6_Haemin -.-> CHEBI_50385
    ing7_Agar["Agar"]
    CultureMech_001284 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2 G_PER_L
2 Na-acetate
C2H3O2.Na 82.034 g/mol
10 G_PER_L CHEBI:32954
sodium acetate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.66 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
7 Haemin
C34H32ClFeN4O4 651.952 g/mol
0.01 G_PER_L CHEBI:50385
hemin
8 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 8.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.424694Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 192
2026-03-13T06:19:02.798535Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001284
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.656974+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PILOBOLUS MEDIUM" to "pilobolus_medium" for consistent matching
2026-06-05T07:41:41.868507+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.220156+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.