Overview
- External ontology
- mediadive.medium:194a (source link unavailable) — DESULFOVIRGA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194a.pdf
Composition
flowchart LR
CultureMech_001289["desulfovirga_medium"]
ing0_Na2SO4["Na2SO4"]
CultureMech_001289 --> ing0_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing0_Na2SO4 -.-> CHEBI_32149
ing1_KH2PO4["KH2PO4"]
CultureMech_001289 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_001289 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_001289 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001289 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_001289 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001289 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001289 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_001289 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_m_Xylene["m-Xylene"]
CultureMech_001289 --> ing9_m_Xylene
CHEBI_28488["CHEBI:28488"]
ing9_m_Xylene -.-> CHEBI_28488
ing10_2_2_4_4_6_8_8_Heptamethylnonane["2,2,4,4,6,8,8-Heptamethylnonane"]
CultureMech_001289 --> ing10_2_2_4_4_6_8_8_Heptamethylnonane
CHEBI_131383["CHEBI:131383"]
ing10_2_2_4_4_6_8_8_Heptamethylnonane -.-> CHEBI_131383
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001289 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_H2SO4["H2SO4"]
CultureMech_001289 --> ing12_H2SO4
CHEBI_26836["CHEBI:26836"]
ing12_H2SO4 -.-> CHEBI_26836
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001289 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_NaOH["NaOH"]
CultureMech_001289 --> ing14_NaOH
CHEBI_32145["CHEBI:32145"]
ing14_NaOH -.-> CHEBI_32145
ing15_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001289 --> ing15_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing15_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001289 --> ing16_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing17_HCl["HCl"]
CultureMech_001289 --> ing17_HCl
CHEBI_17883["CHEBI:17883"]
ing17_HCl -.-> CHEBI_17883
ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001289 --> ing18_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
ing19_ZnCl2["ZnCl2"]
CultureMech_001289 --> ing19_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing19_ZnCl2 -.-> CHEBI_49976
ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001289 --> ing20_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
ing21_H3BO3["H3BO3"]
CultureMech_001289 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001289 --> ing22_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
ing23_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001289 --> ing23_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing23_CuCl2_x_2_H2O -.-> CHEBI_86318
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001289 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001289 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_001289 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001289 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_D_____biotin["D-(+)-biotin"]
CultureMech_001289 --> ing28_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing28_D_____biotin -.-> CHEBI_15956
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_001289 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_001289 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.50215 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.214592 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.321888 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1.07296 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.429185 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.536481 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.160944 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000536481 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
m-Xylene
C8H10 106.168 g/mol
|
0.3 G_PER_L |
CHEBI:28488
m-xylene
|
|
| 11 |
2,2,4,4,6,8,8-Heptamethylnonane
C16H34 226.448 g/mol
|
20 G_PER_L |
CHEBI:131383
2,2,4,4,6,8,8-heptamethylnonane
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
80 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
H2SO4
H2O4S 98.079 g/mol
|
10 G_PER_L |
CHEBI:26836
sulfuric acid
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
40 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 16 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 17 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 18 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 19 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 20 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 21 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 24 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 27 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 33 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min ), then distributed under the same gas atmosphere in anoxic serum vials (e.g., 50 ml medium in 100 ml serum bottles) and autoclaved. Solutions B, D and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions E and F are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to G are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 7.2 - 7.4.
- Note: For transfers use 5 - 10% (v/v) inoculum. Incubate tubes in a slanted position.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.444052Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 194a |
| 2026-03-13T06:19:02.875204Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001289 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.735998+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIRGA MEDIUM" to "desulfovirga_medium" for consistent matching |
| 2026-05-20T06:01:15.045997+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4} |
| 2026-06-05T07:40:58.076520+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.140103+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-12T00:00:00-07:00 | repair_dsmz_194_desulfobulbus_score15.py | RESOLVED_DSMZ_KOMODO_194_SCORE15 | Removed erroneous SOURCE_DUPLICATE variant metadata that linked DSMZ Medium 194a Desulfovirga Medium to KOMODO Medium 194. |