Overview
- External ontology
- mediadive.medium:1c (source link unavailable) — Nutrient bromothymol blue agar NBTA
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001299["nutrient_bromothymol_blue_agar_nbta"]
ing0_Peptone["Peptone"]
CultureMech_001299 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_001299 --> ing1_Meat_extract
ing2_Agar["Agar"]
CultureMech_001299 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Bromothymol_blue["Bromothymol blue"]
CultureMech_001299 --> ing3_Bromothymol_blue
CHEBI_86155["CHEBI:86155"]
ing3_Bromothymol_blue -.-> CHEBI_86155
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Meat extract | 3 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 4 |
Bromothymol blue
C27H28Br2O5S 624.391 g/mol
|
25 MG_PER_L |
CHEBI:86155
bromothymol blue
|
Preparation
- Autoclave at 121 °C for 21 min.
- After autoclavation add 4 mL from a sterile 2,3,5-triphenyltetrazolium chloride stock solution (1% w/v).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:09.958623Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1c |
| 2026-03-13T06:19:02.990748Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001299 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.863722+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient bromothymol blue agar NBTA" to "nutrient_bromothymol_blue_agar_nbta" for consistent matching |
| 2026-06-05T07:41:40.156949+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |