eubacterium_lentum_medium

Overview

External ontology

mediadive.medium:209 (source link unavailable) — EUBACTERIUM LENTUM MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium209.pdf

Composition

flowchart LR
    CultureMech_001307["eubacterium_lentum_medium"]
    ing0_Arginine["Arginine"]
    CultureMech_001307 --> ing0_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing0_Arginine -.-> CHEBI_29016
    ing1_Ground_beef["Ground beef"]
    CultureMech_001307 --> ing1_Ground_beef
    ing2_NaOH["NaOH"]
    CultureMech_001307 --> ing2_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing2_NaOH -.-> CHEBI_32145
    ing3_Casitone["Casitone"]
    CultureMech_001307 --> ing3_Casitone
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001307 --> ing4_Yeast_extract
    ing5_K2HPO4["K2HPO4"]
    CultureMech_001307 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_001307 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_001307 --> ing7_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing7_L_Cysteine_HCl -.-> CHEBI_91247
    ing8_Agar["Agar"]
    CultureMech_001307 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Haemin["Haemin"]
    CultureMech_001307 --> ing9_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing9_Haemin -.-> CHEBI_50385
    ing10_Vitamin_K3["Vitamin K3"]
    CultureMech_001307 --> ing10_Vitamin_K3
    CHEBI_28869["CHEBI:28869"]
    ing10_Vitamin_K3 -.-> CHEBI_28869
    ing11_Ethanol["Ethanol"]
    CultureMech_001307 --> ing11_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing11_Ethanol -.-> CHEBI_16236
    ing12_Vitamin_K1["Vitamin K1"]
    CultureMech_001307 --> ing12_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing12_Vitamin_K1 -.-> CHEBI_18067

Ingredients (13)

Preparation

1. To medium 78 add 0.5% (w/v) arginine.
2. To make medium anoxic bring it to a boil, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under 100% N2 gas atmosphere by filling 7 ml medium into anoxic Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.
3. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving:
4. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add:
5. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
6. Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.
7. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Target organisms

No target organisms are recorded.

Provenance