Overview
- External ontology
- mediadive.medium:210 (source link unavailable) — HALOANAEROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium210.pdf
Composition
flowchart LR
CultureMech_001308["haloanaerobium_medium"]
ing0_NaCl["NaCl"]
CultureMech_001308 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001308 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KCl["KCl"]
CultureMech_001308 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Yeast_extract["Yeast extract"]
CultureMech_001308 --> ing3_Yeast_extract
ing4_Trypticase["Trypticase"]
CultureMech_001308 --> ing4_Trypticase
ing5_Glucose["Glucose"]
CultureMech_001308 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_NaOH["NaOH"]
CultureMech_001308 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
ing7_Na_thioglycolate["Na-thioglycolate"]
CultureMech_001308 --> ing7_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing7_Na_thioglycolate -.-> CHEBI_86481
ing8_Na_ascorbate["Na-ascorbate"]
CultureMech_001308 --> ing8_Na_ascorbate
CHEBI_113451["CHEBI:113451"]
ing8_Na_ascorbate -.-> CHEBI_113451
ing9_Biotin["Biotin"]
CultureMech_001308 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_001308 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001308 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Thiamine_HCl["Thiamine HCl"]
CultureMech_001308 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Riboflavin["Riboflavin"]
CultureMech_001308 --> ing13_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing13_Riboflavin -.-> CHEBI_17015
ing14_Nicotinic_acid["Nicotinic acid"]
CultureMech_001308 --> ing14_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing14_Nicotinic_acid -.-> CHEBI_15940
ing15_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001308 --> ing15_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_Calcium_D_____pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_001308 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001308 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001308 --> ing18__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing18__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001308 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001308 --> ing20_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2_x_4_H2O -.-> CHEBI_86249
ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001308 --> ing21_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001308 --> ing22_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
ing23_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001308 --> ing23_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing23_CaCl2_x_2_H2O -.-> CHEBI_86158
ing24_ZnCl2["ZnCl2"]
CultureMech_001308 --> ing24_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing24_ZnCl2 -.-> CHEBI_49976
ing25_CuCl2["CuCl2"]
CultureMech_001308 --> ing25_CuCl2
CHEBI_49553["CHEBI:49553"]
ing25_CuCl2 -.-> CHEBI_49553
ing26_H3BO3["H3BO3"]
CultureMech_001308 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001308 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001308 --> ing28_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
ing29_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001308 --> ing29_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing29_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
122.495 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 121.495, 1.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
8.2243 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.934579 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 | Yeast extract | 9.34579 G_PER_L | ||
| 5 | Trypticase | 9.34579 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 |
NaOH
HO.Na 39.997 g/mol
|
10 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 8 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
20 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 9 |
Na-ascorbate
C6H7O6.Na 198.106 g/mol
|
20 G_PER_L |
CHEBI:113451
sodium ascorbate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 14 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 15 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 16 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 22 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 23 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 24 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 25 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 26 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.02 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 29 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 30 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Prepare the medium anaerobically under 100% N2. After autoclaving add from sterile solutions:
- Final pH: 9.0.
- Filter sterilize.
- First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.511508Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 210 |
| 2026-01-28T19:48:36.970703Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:48.085525Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:33.549113Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:03.097513Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001308 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.982617+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOANAEROBIUM MEDIUM" to "haloanaerobium_medium" for consistent matching |
| 2026-06-05T07:41:07.439849+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.418508+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/4/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |