Overview
- External ontology
- mediadive.medium:219 (source link unavailable) — MYCOBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium219.pdf
Composition
flowchart LR
CultureMech_001321["mycobacterium_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001321 --> ing0_Yeast_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_001321 --> ing1_Proteose_peptone_no__3
ing2_Casein_peptone["Casein peptone"]
CultureMech_001321 --> ing2_Casein_peptone
ing3_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001321 --> ing3_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing4_KH2PO4["KH2PO4"]
CultureMech_001321 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Sodium_citrate["Sodium citrate"]
CultureMech_001321 --> ing5_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Sodium_citrate -.-> CHEBI_53258
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001321 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Glycerol["Glycerol"]
CultureMech_001321 --> ing7_Glycerol
CHEBI_17754["CHEBI:17754"]
ing7_Glycerol -.-> CHEBI_17754
ing8_Tween["Tween"]
CultureMech_001321 --> ing8_Tween
ing9_Agar["Agar"]
CultureMech_001321 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 2 G_PER_L | ||
| 3 | Casein peptone | 2 G_PER_L | ||
| 4 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
1.5 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
Glycerol
C3H8O3 92.094 g/mol
|
50 G_PER_L |
CHEBI:17754
glycerol
|
|
| 9 | Tween | 0.5 G_PER_L | ||
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.541959Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 219 |
| 2026-03-13T06:19:03.188916Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001321 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.084420+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MYCOBACTERIUM MEDIUM" to "mycobacterium_medium" for consistent matching |
| 2026-06-05T07:41:39.022108+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.336884+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |