sp_medium

CultureMech:001326 bacterial COMPLEX SOLID_AGAR
Original name: SP - MEDIUM

Overview

External ontology
mediadive.medium:222 (source link unavailable) — SP - MEDIUM
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium222.pdf

Composition

flowchart LR
    CultureMech_001326["sp_medium"]
    ing0_Raffinose["Raffinose"]
    CultureMech_001326 --> ing0_Raffinose
    CHEBI_16634["CHEBI:16634"]
    ing0_Raffinose -.-> CHEBI_16634
    ing1_Sucrose["Sucrose"]
    CultureMech_001326 --> ing1_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing1_Sucrose -.-> CHEBI_17992
    ing2_Galactose["Galactose"]
    CultureMech_001326 --> ing2_Galactose
    CHEBI_28260["CHEBI:28260"]
    ing2_Galactose -.-> CHEBI_28260
    ing3_Starch["Starch"]
    CultureMech_001326 --> ing3_Starch
    CHEBI_28017["CHEBI:28017"]
    ing3_Starch -.-> CHEBI_28017
    ing4_Casitone["Casitone"]
    CultureMech_001326 --> ing4_Casitone
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001326 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001326 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Agar["Agar"]
    CultureMech_001326 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Raffinose
C18H32O16 504.438 g/mol
1 G_PER_L CHEBI:16634
raffinose
2 Sucrose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17992
sucrose
3 Galactose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:28260
galactose
4 Starch 5 G_PER_L CHEBI:28017
starch
5 Casitone 2.5 G_PER_L
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.25 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.548076Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 222
2026-03-13T06:19:03.210498Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001326
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.108042+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SP - MEDIUM" to "sp_medium" for consistent matching
2026-06-05T07:41:46.143719+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.573703+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.