polyangium_medium

CultureMech:001327 bacterial DEFINED SOLID_AGAR
Original name: POLYANGIUM MEDIUM

Overview

External ontology
mediadive.medium:223 (source link unavailable) — POLYANGIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium223.pdf

Composition

flowchart LR
    CultureMech_001327["polyangium_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_001327 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001327 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001327 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001327 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001327 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_Agar["Agar"]
    CultureMech_001327 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Tap_water["Tap water"]
    CultureMech_001327 --> ing6_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Tap_water -.-> CHEBI_15377

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
2 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.13 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.032 G_PER_L CHEBI:86254
iron trichloride hexahydrate
6 Agar 10 G_PER_L CHEBI:2509
agar
7 Tap water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water

Preparation

  1. Adjust pH to 7.2 (use pH indicator-paper).
  2. Aseptically add one or more sterile strips of filter paper to slants or plates. Inoculate the filter papers with a small (5 mm x 5mm) agar cube or a piece of filter paper overgrown with the strain.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.549459Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 223
2026-03-13T06:19:03.215205Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001327
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.113285+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "POLYANGIUM MEDIUM" to "polyangium_medium" for consistent matching
2026-06-05T07:41:42.307404+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.758014+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:10.339590+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.