Overview
- External ontology
- mediadive.medium:237 (source link unavailable) — ENB MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium237.pdf
Composition
flowchart LR
CultureMech_001339["enb_medium"]
ing0_Nutrient_broth["Nutrient broth"]
CultureMech_001339 --> ing0_Nutrient_broth
ing1_Peptone["Peptone"]
CultureMech_001339 --> ing1_Peptone
ing2_KH2PO4["KH2PO4"]
CultureMech_001339 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_001339 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_NaCl["NaCl"]
CultureMech_001339 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Glucose["Glucose"]
CultureMech_001339 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar["Agar"]
CultureMech_001339 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nutrient broth | 8 G_PER_L | ||
| 2 | Peptone | 5 G_PER_L | ||
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 6.8 - 7.0. Sterilize glucose separately.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.575206Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 237 |
| 2026-03-13T06:19:03.285842Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001339 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.192841+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ENB MEDIUM" to "enb_medium" for consistent matching |
| 2026-05-20T06:01:16.199812+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:40:59.538649+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.700858+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |