diethyl_phosphonate_medium

CultureMech:001341 bacterial DEFINED SOLID_AGAR
Original name: DIETHYL PHOSPHONATE MEDIUM

Overview

External ontology
mediadive.medium:239 (source link unavailable) — DIETHYL PHOSPHONATE MEDIUM
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium239.pdf

Composition

flowchart LR
    CultureMech_001341["diethyl_phosphonate_medium"]
    ing0_KCl["KCl"]
    CultureMech_001341 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001341 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001341 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Tris_hydroxymethyl_methylamine["Tris(hydroxymethyl)methylamine"]
    CultureMech_001341 --> ing3_Tris_hydroxymethyl_methylamine
    CHEBI_9754["CHEBI:9754"]
    ing3_Tris_hydroxymethyl_methylamine -.-> CHEBI_9754
    ing4_p_Hydroxybenzoate["p-Hydroxybenzoate"]
    CultureMech_001341 --> ing4_p_Hydroxybenzoate
    CHEBI_17879["CHEBI:17879"]
    ing4_p_Hydroxybenzoate -.-> CHEBI_17879
    ing5_Agar["Agar"]
    CultureMech_001341 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Diethyl_phosphonate["Diethyl phosphonate"]
    CultureMech_001341 --> ing6_Diethyl_phosphonate

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 KCl
Cl.K 74.551 g/mol
0.2 G_PER_L CHEBI:32588
potassium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.2 G_PER_L CHEBI:31206
ammonium chloride
4 Tris(hydroxymethyl)methylamine
C4H11NO3 121.136 g/mol
6 G_PER_L CHEBI:9754
tris
5 p-Hydroxybenzoate
C7H5O3 137.114 g/mol
0.75 G_PER_L CHEBI:17879
4-hydroxybenzoate
6 Agar 12 G_PER_L CHEBI:2509
agar
7 Diethyl phosphonate 100 G_PER_L

Preparation

  1. Adjust pH to 7.4. After sterilization add 100 ml of a filter-sterilized solution of 1 mM diethyl phosphonate (Eastman Organic Chemical Div., Rochester, N.Y., USA) or of 20 mM phosphate buffer.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.577623Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 239
2026-03-13T06:19:03.292683Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001341
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.201147+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DIETHYL PHOSPHONATE MEDIUM" to "diethyl_phosphonate_medium" for consistent matching
2026-06-05T07:40:58.702817+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.378558+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.