Overview
- External ontology
- mediadive.medium:242 (source link unavailable) — BMM - MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium242.pdf
Composition
flowchart LR
CultureMech_001344["bmm_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001344 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_001344 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_001344 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaCl["NaCl"]
CultureMech_001344 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001344 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Yeast_extract["Yeast extract"]
CultureMech_001344 --> ing5_Yeast_extract
ing6_Biotin["Biotin"]
CultureMech_001344 --> ing6_Biotin
CHEBI_15956["CHEBI:15956"]
ing6_Biotin -.-> CHEBI_15956
ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001344 --> ing7_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing8_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001344 --> ing8_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing8_MnSO4_x_H2O -.-> CHEBI_86364
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001344 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_Methanol["Methanol"]
CultureMech_001344 --> ing10_Methanol
CHEBI_17790["CHEBI:17790"]
ing10_Methanol -.-> CHEBI_17790
ing11_Agar["Agar"]
CultureMech_001344 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
7 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 | Yeast extract | 0.1 G_PER_L | ||
| 7 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 8 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.0001 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 9 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0062 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
Methanol
CH4O 32.042 g/mol
|
10 G_PER_L |
CHEBI:17790
methanol
|
|
| 12 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
Preparation
- Adjust pH to 7.2 prior to autoclaving.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.581386Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 242 |
| 2026-03-13T06:19:03.304953Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001344 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.215575+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BMM - MEDIUM" to "bmm_medium" for consistent matching |
| 2026-06-05T07:40:51.259416+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.938725+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |