Overview
- External ontology
- mediadive.medium:246a (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM 246
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium246a.pdf
Composition
flowchart LR
CultureMech_001347["reactivation_with_liquid_medium_246"]
ing0_Beef_extract["Beef extract"]
CultureMech_001347 --> ing0_Beef_extract
ing1_Peptone["Peptone"]
CultureMech_001347 --> ing1_Peptone
ing2_Agar["Agar"]
CultureMech_001347 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Tap_water["Tap water"]
CultureMech_001347 --> ing3_Tap_water
CHEBI_15377["CHEBI:15377"]
ing3_Tap_water -.-> CHEBI_15377
ing4_Sea_water["Sea water"]
CultureMech_001347 --> ing4_Sea_water
ing5_NaCl["NaCl"]
CultureMech_001347 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_KCl["KCl"]
CultureMech_001347 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001347 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001347 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_NaHCO3["NaHCO3"]
CultureMech_001347 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001347 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Beef extract | 10 G_PER_L | ||
| 2 | Peptone | 10 G_PER_L | ||
| 3 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
Tap water
H2O 18.015 g/mol
|
250 G_PER_L |
CHEBI:15377
water
|
|
| 5 | Sea water | 750 G_PER_L | ||
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
28.13 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.77 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.11 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Preparation
- Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature.
- Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.
- Rehydrate and grow lyophilized cells from the ampoule in liquid medium 246. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.587789Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 246a |
| 2026-03-13T06:19:03.320095Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001347 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.232674+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "REACTIVATION WITH LIQUID MEDIUM 246" to "reactivation_with_liquid_medium_246" for consistent matching |
| 2026-06-05T07:41:44.081899+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |