peptone_meat_extract_soil_extract_agar_pfe

CultureMech:001350 bacterial COMPLEX SOLID_AGAR
Original name: PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE)

Overview

External ontology
mediadive.medium:251 (source link unavailable) — PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium251.pdf

Composition

flowchart LR
    CultureMech_001350["peptone_meat_extract_soil_extract_agar_pfe"]
    ing0_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_001350 --> ing0_Proteose_peptone_no__3
    ing1_Meat_extract["Meat extract"]
    CultureMech_001350 --> ing1_Meat_extract
    ing2_Glycerol["Glycerol"]
    CultureMech_001350 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_Soil_extract["Soil extract"]
    CultureMech_001350 --> ing3_Soil_extract
    ing4_Agar["Agar"]
    CultureMech_001350 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Proteose peptone no. 3 5 G_PER_L MICRO:0000180 (source link unavailable)
Proteose Peptone
2 Meat extract 3 G_PER_L
3 Glycerol
C3H8O3 92.094 g/mol
20 G_PER_L CHEBI:17754
glycerol
4 Soil extract 150 G_PER_L MICRO:0000457 (source link unavailable)
Soil extract
5 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.593567Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 251
2026-03-13T06:19:03.331976Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001350
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.246100+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE)" to "peptone_meat_extract_soil_extract_agar_pfe" for consistent matching
2026-06-05T07:41:41.317855+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch7.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 Applied local mappings for Proteose peptone no. 3, Soil extract.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.