bdellovibrio_yp_medium

Overview

External ontology

mediadive.medium:257 (source link unavailable) — BDELLOVIBRIO (YP) MEDIUM

Applications

• Microbial cultivation

pH

7.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium257.pdf

Composition

flowchart LR
    CultureMech_001356["bdellovibrio_yp_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001356 --> ing0_Yeast_extract
    ing1_Peptone["Peptone"]
    CultureMech_001356 --> ing1_Peptone
    ing2_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_001356 --> ing2_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing2_Tris_HCl_buffer -.-> CHEBI_9754

Ingredients (3)

Preparation

1. Preparation of double layered agar plates:<p> <b>Bottom layer:</b> Dissolve 12.0 g Bacto agar in 900 ml distilled water and autoclave. After cooling to 60°C add 100 ml of sterile YP medium (autoclaved at 121°C for 15 min) and pour plates. Dry plates at room temperature for 2 - 3 days .<p> <b>Top layer:</b> Grow prey bacterium on agar slants using medium 54. Prepare 5 ml aliquots of 0.6% Bacto agar in distilled water and autoclave in test tubes. After cooling to 45°C in a water bath add 0.5 ml sterile YP medium (autoclaved at 121°C for 15 min) and 0.5 ml cell suspension of host organism, mix and pour top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.<p>
2. Inoculate double layer plates the same day when prepared.

Target organisms

No target organisms are recorded.

Provenance