medium_for_chemolithotrophic_growth_of_bacillus_schlegelii

CultureMech:001360 bacterial DEFINED SOLID_AGAR
Original name: MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII

Overview

External ontology
mediadive.medium:261 (source link unavailable) — MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium261.pdf

Composition

flowchart LR
    CultureMech_001360["medium_for_chemolithotrophic_growth_of_bacillus_schlegelii"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001360 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001360 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001360 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001360 --> ing3_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing3_MnSO4_x_H2O -.-> CHEBI_86364
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001360 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001360 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_001360 --> ing6_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing7_Agar["Agar"]
    CultureMech_001360 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Distilled_water["Distilled water"]
    CultureMech_001360 --> ing8_Distilled_water

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
4.5 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.01 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Ferric ammonium citrate 0.005 G_PER_L CHEBI:31604
ferric ammonium citrate
8 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
9 Distilled water 1 L mediadive.compound:4 (source link unavailable)
Distilled water

Preparation

  1. Adjust pH to 7.1. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C.
  2. Incubate at 65C without agitation under 5% (v/v) O2, 10% CO2, 45% H2

Solutions

Trace element solution SL-6

Dose: 0.003 L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 0.1 G_PER_L
MnCl2 x 4 H2O 0.03 G_PER_L
H3BO3 0.3 G_PER_L
CoCl2 x 6 H2O 0.2 G_PER_L
CuCl2 x 2 H2O 0.01 G_PER_L
NiCl2 x 6 H2O 0.02 G_PER_L
Na2MoO4 x 2 H2O 0.03 G_PER_L
Distilled water 1 L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.610202Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 261
2026-03-13T06:19:03.390945Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001360
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.313582+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII" to "medium_for_chemolithotrophic_growth_of_bacillus_schlegelii" for consistent matching
2026-06-05T07:41:28.937899+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.009372+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T11:35:03.558874+00:00 codex RESTORED_REVIEWED_MEDIUM_STOCK_BOUNDARIES Exact source graph, complete ingredient inventory, amounts, and original PDF recipe chain checked. Numeric concentrations and chemical mappings preserved; stock doses and water are explicit batch amounts. No final-medium stock dilution inferred. Parent/variant relationships remain separately unreviewed.