j_agar

CultureMech:001361 bacterial COMPLEX SOLID_AGAR
Original name: J - AGAR

Overview

External ontology
mediadive.medium:262 (source link unavailable) — J - AGAR
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium262.pdf

Composition

flowchart LR
    CultureMech_001361["j_agar"]
    ing0_Tryptone["Tryptone"]
    CultureMech_001361 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001361 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_001361 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001361 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Agar["Agar"]
    CultureMech_001361 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Tryptone 5 G_PER_L
2 Yeast extract 15 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
4 K2HPO4
HO4P.2K 174.174 g/mol
3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.3 - 7.5. Dissolve glucose in 10 ml of distilled water and sterilize glucose separately. Aseptically add to the basis medium after sterilization.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.612621Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 262
2026-03-13T06:19:03.395560Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001361
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.319396+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "J - AGAR" to "j_agar" for consistent matching
2026-05-20T06:01:23.404371+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5}
2026-06-05T07:41:09.731277+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.162185+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.