acidiphilium_medium

Overview

External ontology

mediadive.medium:269 (source link unavailable) — ACIDIPHILIUM MEDIUM

Applications

• Microbial cultivation

pH

3.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf

Composition

flowchart LR
    CultureMech_001368["acidiphilium_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001368 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KCl["KCl"]
    CultureMech_001368 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001368 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001368 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001368 --> ing4_Yeast_extract
    ing5_D_Glucose["D-Glucose"]
    CultureMech_001368 --> ing5_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing5_D_Glucose -.-> CHEBI_17634

Ingredients (6)

Preparation

1. Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Acidiphilium cryptum

  Term

  Id

  NCBITaxon:524

  Label

  Acidiphilium cryptum

  Growth metrics

  • Doubling time minutes

    312.0

    Ph at measurement

    3.0

    Evidence

    • Reference

      PMID:12420179

      Supports

      SUPPORT

      Snippet

      with a doubling time of 7.6 h, as compared to 5.2 h of growth without aluminum

      Explanation

      Auto-applied from literature query: targeted: Acidiphilium cryptum doubling time

    Is max attainment

    True

Recipe variants

• Name

  aluminum_sulfate_stress_acidiphilium_cryptum

  Description

  Acidiphilium cryptum growth in acidic glucose-mineral medium under aluminum sulfate stress.

  Modifications

  • Maintain pH 3.0.
  • Add aluminum sulfate at the stress concentration reported by the source study.
  • Use Acidiphilium cryptum as the target organism.

  Purpose

  Capture aluminum-stress growth evidence under the parent Acidiphilium Medium record.

  Evidence

  • Reference

    PMID:12420179

    Supports

    SUPPORT

    Snippet

    with a doubling time of 7.6 h, as compared to 5.2 h of growth without aluminum

    Explanation

    Literature evidence supports modeling the aluminum sulfate growth condition as a variant of the parent Acidiphilium Medium record.

Provenance