cherry_agar_cbs_formula

CultureMech:001374 bacterial COMPLEX SOLID_AGAR
Original name: CHERRY AGAR (CBS formula)

Overview

External ontology
mediadive.medium:277 (source link unavailable) — CHERRY AGAR (CBS formula)
Applications
  • Microbial cultivation
pH range
{'min': 3.8, 'max': 4.6}
Notes
DSMZ Medium 277 prepares CHERRY AGAR (CBS formula) from a cherry extract made by simmering 200.0 g pulp of sour stone cherries in 1 l water, then adds 200 ml sterilized cherry extract to 20.0 g agar dissolved in 800 ml water. Final pH is 3.8-4.6.

Composition

flowchart LR
    CultureMech_001374["cherry_agar_cbs_formula"]
    ing0_Agar["Agar"]
    CultureMech_001374 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Distilled_water["Distilled water"]
    CultureMech_001374 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Agar 20.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 277 dissolves 20.0 g agar in the final agar base.
2 Distilled water
H2O 18.015 g/mol
800.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 277 dissolves agar in 800 ml water before adding cherry extract.

Preparation

  1. Add 1 l water to 200.0 g pulp of sour stone cherries, heat to boiling, simmer gently for 2 h, and strain through cloth.
  2. Sterilize the cherry extract at 110 C for 30 min.
  3. Dissolve 20.0 g agar in 800 ml water and sterilize.
  4. Add 200 ml cherry extract, distribute in presterilized tubes, and sterilize for 5 min at 120 C.

Solutions

Cherry extract

Dose: 200.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Pulp of sour stone cherries 200.0 G_PER_L DSMZ Medium 277 extracts 200.0 g pulp of sour stone cherries in water.
Distilled water 1000.0 ML_PER_L DSMZ Medium 277 adds 1 l water while preparing the cherry extract.
DSMZ Medium 277 adds 200 ml sterilized cherry extract.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.635102Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 277
2026-03-13T06:19:03.475148Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001374
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.408050+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CHERRY AGAR (CBS formula)" to "cherry_agar_cbs_formula" for consistent matching
2026-05-20T06:01:11.395228+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '3.8-4.6' -> {'min': 3.8, 'max': 4.6}
2026-06-05T07:40:53.246329+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_dsmz_277_cherry_agar_score15.py RESOLVED_DSMZ_277_CHERRY_AGAR_SCORE15 DSMZ Medium 277 prepares CHERRY AGAR (CBS formula) from a cherry extract made by simmering 200.0 g pulp of sour stone cherries in 1 l water, then adds 200 ml sterilized cherry extract to 20.0 g agar dissolved in 800 ml water. Final pH is 3.8-4.6.