rhodospirillaceae_medium_modified

CultureMech:001375 bacterial COMPLEX LIQUID
Original name: RHODOSPIRILLACEAE MEDIUM (modified)

Overview

External ontology
mediadive.medium:27 (source link unavailable) — RHODOSPIRILLACEAE MEDIUM (modified)
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf Composition verified against https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf on 2026-08-25 (SHA-256 e9c7d7ca3bfea7e2300e746a51320e5b47b4f6aa2237abe840b01471af25d9a9).

Composition

flowchart LR
    CultureMech_001375["rhodospirillaceae_medium_modified"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001375 --> ing0_Yeast_extract
    ing1_Disodium_succinate["Disodium succinate"]
    CultureMech_001375 --> ing1_Disodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing1_Disodium_succinate -.-> CHEBI_63675
    ing2_Ammonium_acetate["Ammonium acetate"]
    CultureMech_001375 --> ing2_Ammonium_acetate
    CHEBI_62947["CHEBI:62947"]
    ing2_Ammonium_acetate -.-> CHEBI_62947
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001375 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001375 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_001375 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_NH4Cl["NH4Cl"]
    CultureMech_001375 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001375 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_001375 --> ing8_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing8_L_Cysteine_HCl -.-> CHEBI_91247
    ing9_Distilled_water["Distilled water"]
    CultureMech_001375 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.30 G_PER_L FOODON:03315426
yeast extract
2 Disodium succinate
C4H4O4.2Na 162.052 g/mol
1.00 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
3 Ammonium acetate
C2H3O2.H4N 77.083 g/mol
0.50 G_PER_L CHEBI:62947
ammonium acetate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.40 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NaCl
Cl.Na 58.443 g/mol
0.40 G_PER_L CHEBI:26710
sodium chloride
7 NH4Cl
Cl.H4N 53.492 g/mol
0.40 G_PER_L CHEBI:31206
ammonium chloride
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
0.30 G_PER_L CHEBI:91247
L-cysteine hydrochloride
10 Distilled water
H2O 18.015 g/mol
1000.00 ML_PER_L CHEBI:15377
water
 Amount printed for the final-medium batch in DSMZ Medium 27.

Preparation

  1. Adjust pH to 6.8.
  2. Boil for a few minutes. Bubble with nitrogen, dispense 10 ml into 15 ml tubes with rubber septa under nitrogen, and autoclave at 121 C for 15 minutes. Use sterile syringes for inoculation and sampling.
  3. Incubate in the light using a tungsten lamp.

Solutions

Fe(III) citrate solution (0.1% in water)

Dose: 5.00 ML_PER_L

Stock composition
IngredientConcentrationNotes
Fe(III) citrate 1.00 G_PER_L
Distilled water 1000.00 ML_PER_L
0.1% (w/v) stock; add 5.00 ml per printed final-medium batch.

Vitamin B12 solution (10 mg in 100 ml water)

Dose: 0.40 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.10 G_PER_L
Distilled water 1000.00 ML_PER_L
10 mg per 100 ml stock; add 0.40 ml per printed final-medium batch. This is 0.04 mg vitamin B12 per litre before any volume correction.

Trace element solution SL-6

Dose: 1.00 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 0.10 G_PER_L
MnCl2 x 4 H2O 0.03 G_PER_L
H3BO3 0.30 G_PER_L
CoCl2 x 6 H2O 0.20 G_PER_L MIM SSSOM maps this hydrated source label to cobalt dichloride.
CuCl2 x 2 H2O 0.01 G_PER_L
NiCl2 x 6 H2O 0.02 G_PER_L MIM SSSOM maps this hydrated source label to nickel dichloride.
Na2MoO4 x 2 H2O 0.03 G_PER_L
Distilled water 1000.00 ML_PER_L
DSMZ SL-6 stock composition is printed per litre; add 1.00 ml.

Resazurin solution (0.1%)

Dose: 0.50 ML_PER_L

Stock composition
IngredientConcentrationNotes
Resazurin 1.00 G_PER_L
Distilled water 1000.00 ML_PER_L
0.1% (w/v) stock; add 0.50 ml per printed final-medium batch.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:09.992377Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 27
2026-03-13T06:19:03.482536Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001375
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.416027+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RHODOSPIRILLACEAE MEDIUM (modified)" to "rhodospirillaceae_medium_modified" for consistent matching
2026-06-05T07:41:44.419499+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.992805+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-25T00:00:00-07:00 repair_dsmz_27_44_compositions.py CORRECTED_DSMZ_27_44_STOCK_STRUCTURE Restored the reviewed DSMZ Medium 27 formulation while preserving four stock-solution boundaries. Corrected vitamin B12, SL-6, and resazurin concentration semantics; selected identities were verified in the MIM SSSOM.